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. 2016 Aug 16;7(37):58832–58847. doi: 10.18632/oncotarget.11315

Figure 6. Red fluorescence sperm was detected during incubation with different concentration of DiI-exo (DiI-exo-4 and DiI-exo-16) at 17°C.

Figure 6

The sperm samples were treated by 0.125% trypsin and then washed 3 times using the diluent. Finally, the 2 μL of sperm samples were dropped on cover slides and observed using fluorescence microscopy. A. Sperm was incubated one day in the diluent with DiI-exo-4; B. Sperm was incubated one day in the diluent with DiI-exo-16; C. Sperm was incubated 4 days in the diluent with DiI-exo-4; D. Sperm was incubated 4 days in the diluent with DiI-exo-16. E. Sperm incubated 3 hours in the diluent with 0.005 mM DiI dye displayed red fluorescence. DiI-exo-4, DiI-exo-16 represented the diluent with four and sixteen times concentration of exosomes of original semen, respectively. And the exosomes were labeled DiI dye. Scale: sperm head length 7.0 μm.