Table 2. Sequencing characteristics of genomic single-and double-strand libraries.
| Sample No. | Library type | Library ID | Mergetrimmed reads | Mapped reads | % | Unique reads | % | Mapped reads of unique | % | Molecules in library | Median molecule length (bp) | Mappable bp | Estimated genome coverage |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ss | A5347 | 1539768 | 1294718 | 84 | 1539768 | 100 | 1282731 | 83 | 2.90E+09 | 76 | 1.84E+11 | 55.64 |
| 2 | ss | A5348 | 1255872 | 1078130 | 86 | 1255872 | 100 | 1070829 | 85 | 5.60E+09 | 58 | 2.77E+11 | 83.92 |
| 3 | ss | A5349 | 1318068 | 1111409 | 84 | 1318068 | 100 | 1102175 | 84 | 4.10E+09 | 81 | 2.78E+11 | 84.15 |
| 4 | ss | A5350 | 1607846 | 1397991 | 87 | 1607846 | 100 | 1390490 | 86 | 6.32E+09 | 62 | 3.39E+11 | 102.69 |
| NTC1 | ss | A5351 | 236065 | 38093 | 16 | 236065 | 100 | 23877 | 10 | 1.79E+08 | n.d. | ||
| 17 | ss | MS1 | 4114005 | 3683777 | 90 | 4036964 | 98 | 3606736 | 89 | 2.60E+09 | 51 | 1.16E+11 | 35.23 |
| 18 | ss | MS2 | 3229456 | 2859033 | 89 | 3176913 | 98 | 2806490 | 88 | 2.04E+09 | 61 | 1.08E+11 | 32.77 |
| 19 | ss | MS3 | 3138691 | 2799387 | 89 | 3101849 | 99 | 2762545 | 89 | 2.72E+09 | 60 | 1.44E+11 | 43.53 |
| 20 | ss | MS4 | 3988112 | 3615532 | 91 | 3959556 | 99 | 3586976 | 91 | 8.25E+09 | 52 | 3.86E+11 | 116.92 |
| 21 | ss | MS5 | 3821539 | 3362766 | 88 | 3738118 | 98 | 3279345 | 88 | 3.19E+09 | 57 | 1.56E+11 | 47.28 |
| 17 | ds | MS1dsMPI | 2868381 | 1623445 | 57 | 2582011 | 90 | 1337075 | 52 | 8.82E+06 | 76 | 3.12E+08 | 0.09 |
| 18 | ds | MS2dsMPI | 3426906 | 1902541 | 56 | 2723388 | 79 | 1199023 | 44 | 6.14E+06 | 86 | 1.85E+08 | 0.06 |
| 19 | ds | MS3dsMPI | 3575035 | 2046427 | 57 | 3339449 | 93 | 1810841 | 54 | 3.66E+07 | 86 | 1.59E+09 | 0.48 |
| 20 | ds | MS4dsMPI | 3400508 | 2065718 | 61 | 3109447 | 91 | 1774657 | 57 | 2.56E+07 | 79 | 1.06E+09 | 0.32 |
| 21 | ds | MS5dsMPI | 3812279 | 2181953 | 57 | 3304041 | 87 | 1673715 | 51 | 1.27E+07 | 82 | 4.57E+08 | 0.14 |
| 17 | ds | MS1NEB | 3910758 | 2194541 | 56 | 2785746 | 71 | 1069529 | 38 | 1.68E+06 | 88 | 4.04E+07 | 0.01 |
| 18 | ds | MS2NEB | 2902274 | 727263 | 25 | 2393922 | 82 | 218911 | 9 | 7.98E+06 | 92 | 5.54E+07 | 0.02 |
| 19 | ds | MS3NEB | 3870670 | 2197359 | 57 | 2369050 | 61 | 695739 | 29 | 6.05E+06 | 99 | 1.08E+08 | 0.03 |
| 20 | ds | MS4NEB | 2788557 | 1631982 | 59 | 1622804 | 58 | 466229 | 29 | 3.23E+06 | 87 | 4.70E+07 | 0.01 |
| 21 | ds | MS5NEB | 3435934 | 1624254 | 47 | 2152694 | 63 | 341014 | 16 | 3.65E+06 | 88 | 3.19E+07 | 0.01 |
The percentages of mapped reads are reported without quality filters and before duplicate read removal as well as thereafter for the remaining unique reads. Ss. Single-strand. Ds. Double-strand. NTC. Negative control.