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. 2016 Aug 3;7(37):59245–59259. doi: 10.18632/oncotarget.11043

Figure 1. FOXM1 counteracts gefitinib-induced cell death of lung adenocarcinoma cells.

Figure 1

(A) PC9/GR and HCC827/GR were more resistant to gefitinib than their parental cells, respectively. (B) Cell proliferation rates were detected using MTT assay for four days, following treatment with gefitinib. (C) PC9, PC9/GR, HCC827 and HCC827/GR cells were transfected with negative control shRNA (shNC), pcDNA3.1 control vector (pcDNA3.1), pcDNA3.1-FOXM1 (FOXM1) or FOXM1 shRNA (shFOXM1) for 48 hrs, then treated with various concentrations of gefitinib for 72 hrs, and cell viability was analyzed using MTT assay. (D) Time-dependent effects of FOXM1 on the proliferation of PC9, PC9/GR, HCC827 and HCC827/GR cells were confirmed using MTT assay, following treatment with gefitinib. (E and F) EdU staining for evaluation of the influences of FOXM1 on the proliferation of lung adenocarcinoma cells. Cells were exposed to gefitinib for 72 hrs and subjected to EdU incorporation assays. The new generation cells were detected via EdU (red). DAPI stained nuclei in blue. Merged view of EdU (red) and DAPI (blue) showing the overlap. Each bar represents the mean ± SD. P values were calculated using Student's t-test (*P < 0.05, ** P < 0.01, *** P < 0.001).