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. 2016 Aug 5;7(37):60432–60445. doi: 10.18632/oncotarget.11080

Figure 8. Bod1 overexpression inhibits miR-142-3p-induced premature chromatid separation.

Figure 8

A. Relative Bod1 mRNA expression was measured by qRT-PCR in 786-O and A549 cells after transfection with Bod1 overexpression vector or negative vector (NC). GAPDH was used as the internal control. B. Western blot assay of Bod1 protein level were measured in 786-O and A549 cells after transfection with Bod1 overexpression vector or negative vector (NC). C. Relative Bod1 protein levels were quantified using Image J software. D. Relative Bod1 mRNA expression was measured by qRT-PCR in 786-O and A549 cells after transfection with Bod1 overexpression vector, negative vector, or miR-142-3p and negative mimics. E. Proportion of 786-O and A549 cells with premature chromatid separation after transfection of Bod1 overexpression vector, negative vector, or miR-142-3p and negative mimics. Each data point represents the mean of three separate experiments; bars indicate standard errors. *P < 0.05; **P < 0.01.