Fig. 1.
Maps of novel antibiotic-cassette vectors. The location of antibiotic resistance-conferring genes, bla (ampicillin-resistance), aphA2 (kanamycin-resistance), catA2 (chloramphenicol-resistance) and dfrB2 (trimethoprim-resistance), and the plasmid ColE1 origin of replication (oriR) are indicated for plasmids p34E-Km (top), p34E-Cm2 (left) and p34E-TpTer. Dual-cutting restriction sites that can be utilised to excise the antibiotic resistance cassette are also shown, as are internal sites that can be used for determination of the orientation of the cassette following insertion into a target gene. Restriction sites that occur only once in each plasmid are shown in bold. Note that additional sites for AseI, BsrGI, NheI and XmnI occur in the backbone of all three vectors that for clarity are not shown. Promoters for the cassette antibiotic-resistance genes are shown (Paph, Pcat, PcS). Maps created with SnapGene® software (from GSL Biotech; available at snapgene.com).