Promoter elements and RNA polymerase regions responsible for recognition of each promoter element. A: Regions of core (αCTD and β) and σ70 that make sequence-specific interactions with promoter DNA are indicated above the DNA elements with which they interact. The transcription start site (TSS) is shown as a bent arrow. αCTD (carboxyl-terminal domain of α subunit; -35 (-35 hexamer), Ext (extended -10 element), -10 (-10 hexamer), Dis (discriminator element), CRE (core recognition element). B:
rrnB P1 and consensus sequence for σ70-dependent promoters. rrnB P1 bases identical to the consensus sequence are indicated. Promoter elements are colored as in (A). W=A or T, n= any base. Spacing between -35 and extended -10 and between discriminator element and TSS are indicated by brackets. The number of bp separating elements is indicated.