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. Author manuscript; available in PMC: 2018 Feb 7.
Published in final edited form as: Structure. 2017 Jan 19;25(2):329–340. doi: 10.1016/j.str.2016.12.011

Fig. 4. Dim1 binding joins the head to the body.

Fig. 4

A. Local 3D classification of Dim1 reveals a single class with crescent-shaped density (green) that corresponds well to the X-ray crystal structure of human Dim1 (PDB 1ZQ9, (Dong et al., 2005). By orienting its N-terminus to Rio2, R233-K236 (four red spheres represent each Cα atom position), which are together responsible for binding pre-40S, point to rRNA in the body of the small subunit. Yellow spheres for each SAM atom mark the active site.

B. A second class emerged from 3D classification that lacked Dim1, suggesting this AF is not integral to the integrity of the pre-40S subunit.

C. Growth of galactose-inducible/glucose-repressible Dim1 (GAL::Dim1) yeast cells carrying an empty vector (VO), or vectors coding wild-type (WT) or Dim1_RKNK is compared on glucose and galactose-containing plates.

D. Sucrose gradients of cell extracts from GAL::Dim1 cells transformed with vectors carrying WT-Dim1 or Dim1_RKNK, grown in glucose for 16 h. Shown are absorbance profiles at 254 nm and Western blots for Dim1, Enp1 and Tsr1. See also Figures S2 and S3.