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Three variant positions at different frequencies were amplified by PCR, cloned and Sanger sequenced for validation.
| Position | Frequency | Clones total | Clones supportive | Sanger confirmed |
|---|---|---|---|---|
| 1,338,568 | 28 | 16 | 6 | Yes |
| 1,339,720 | 11 | 16 | 2 | Yes |
| 1,339,224 | 4 | 16 | 1 | Yes |
