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. 2017 Feb 20;3(2):e00250. doi: 10.1016/j.heliyon.2017.e00250

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Lipid peroxidation levels are significantly decreased in WT as well as Pmca4^−/− sperm following administration of the walnut-enriched diet. Lipid peroxidation levels were assessed via a Thiobarbituric Acid Reactive Substances Assay (TBARS) assay. Mean malondialdehyde [MDA (μM)] levels in WT and Pmca4^−/− sperm from control and animals treated with a diet (enriched in PUFAs and antioxidants) for 9–11 weeks are shown. Lipid peroxidation levels decrease in both WT and nulls following diet implementation. (Two-way ANOVA: *p < 0.05, N = 6, mean ± SEM).