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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Hepatology. 2016 Dec 31;65(3):983–998. doi: 10.1002/hep.28921

Figure 6. Profibrotic stimuli induce TANGO1 expression specifically in HSCs.

Figure 6

A. mRNA was harvested from cirrhotic and non-cirrhotic human livers and TANGO1 levels were analyzed by qPCR (9 samples per group, *p<0.05). B and C. Livers were harvested from C57Bl/6J mice that either were treated with CCl4 (or olive oil as a control) or underwent BDL (or a sham surgery). At sacrifice, mRNA was harvested from whole livers and analyzed for TANGO1 mRNA levels by PCR. *=p<0.05. D. Immortalized liver cells (LX-2, HepG2, TSEC, or NHC2) or primary human HSCs were treated with 10ng/ml TGFβ for 24hrs, after which mRNA was extracted and TANGO1 expression was analyzed by qPCR (n=4). *=p<0.01 D. HSCs were treated with TGFβ and analyzed for TANGO1 protein expression by immunoblot (quantification below). n=6, *=p<0.05.