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. Author manuscript; available in PMC: 2017 Jul 11.
Published in final edited form as: Nature. 2017 Jan 11;542(7641):377–380. doi: 10.1038/nature21078

Extended Data Figure 2. Data processing workflow.

Extended Data Figure 2

a, Method used to obtain an initial model of the C* complex for 3D classification in RELION39. b, Scheme for 3D classification and refinement. The mask used to obtain the overall 3.8Å map excluded the Clf1 and Syf1 arch regions as well as the Prp22 and U2 snRNP regions. Note that focused classification was performed without signal subtraction. All nominal resolutions reported were obtained during post-processing in RELION39.