Figure 9. Reversal potentials as a function of various cable properties.
Each lolliplot shows the glutamate response apparent reversal potentials (Erevs) for photo-uncaging sites as function of their distance from the soma, branch order, and neurite diameter. Each color and lolliplot is indicative of the set of reversal potentials across sites within a single neuron (N = 10). Focal glutamate responses were evoked at varying membrane potentials (between −120 and −40 mV) with two-electrode current clamp at the soma. Apparent Erevs for each photo-uncaging site were determined as in Figure 6. Distance and branch order measurements were generated from skeletal reconstructions. Diameter measurements were generated by manual measurement of neuronal dye-fill confocal stacks.
Figure 9—figure supplement 1. Reversal potentials as a function of various cable properties with linear fits.
Each lolliplot shows the glutamate response apparent reversal potentials (Erevs) for photo-uncaging sites as function of their distance from the soma, branch order, and neurite diameter. Each color and lolliplot is indicative of the set of reversal potentials across sites within a single neuron (N = 10). Focal glutamate responses were evoked at varying membrane potential (between −120 and −40 mV) with two-electrode current clamp at the soma. Apparent Erevs for each photo-uncaging site were determined as in Figure 6. Distance and branch. order measurements were generated from skeletal reconstructions. Diameter measurements were generated by manual measurement of neuronal dye-fill confocal stacks. The above plots were fit were linear regression analyses. The resulting linear functions are plotted on the same axes (dashed black lines). Across all 10 preparations and cable properties, slopes were nearly. zero. Statistics for the linear regression analyses are shown in Table 2.