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. 2016 Apr 8;7(40):64766–64777. doi: 10.18632/oncotarget.8649

Figure 2. Knockdown of COX-2 differentially resensitizes DiF5 cells to treatment with cetuximab, AG1478, or gefitinib.

Figure 2

(A) DiFi5 cells were transfected with a COX-2 shRNA. Stable clones were selected and analyzed by Western blot analysis, along with parental DiFi and control vector-transfected DiFi5 cells, using a COX-2-specific antibody. Both short and long exposures of the results are shown. (B) DiFi, DiFi5, and three COX-2 knockdown DiFi5 clones were cultured in 0.5% fetal bovine serum medium containing indicated concentrations of cetuximab, AG1478, or gefitinib for 3 days and then subjected to an MTT assay. The data shown are the optical density (OD) values of untreated and treated cell groups at the end of treatment, expressed as a percentage of the OD value of corresponding untreated or vehicle-treated cells. *p < 0.05 for comparison of the values of DiFi5 COX-2 knockdown clones with the values of DiFi5; ns, not significant.