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. 2016 Aug 19;7(40):65137–65146. doi: 10.18632/oncotarget.11427

Figure 3. Knocking down ELK3 in MDA-MB-231 cells renders them unresponsive to TGF-ß.

Figure 3

(A) Relative expression of TGFß1, TGFß2, TGFß3, TGFR1, and TGFR2 in C1 and KD1 cells. (B) Phosphorylation and nuclear accumulation of Smad2 in C1 and KD1 cells upon TGF-ß treatment were analyzed by immunoblotting and immunocytochemical staining, respectively. (C) Relative expression of Vimentin, Slug, and Snail (target genes of Smad2) in C1 and KD1 cells treated with TGF-ß. Error bars represent the standard error from three independent experiments, each performed using triplicate samples. *P < 0.05 and **P < 0.01 (Student's t-test).