Skip to main content
. 2016 Sep 1;7(40):65504–65513. doi: 10.18632/oncotarget.11798

Figure 1. Cancer cells and BCSCs are clearly sensitized to ionizing radiation (RT) by DNA-PKcs inhibition and hyperthermia (HT).

Figure 1

(A) and (B) Clonogenic assay with increasing radiation dose after combined treatment in SiHa (A) and MCF7 (B) cells. Survival curves were established using the linear quadratic regression model, corresponding α and β values can be found in Supplementary Table S1. (C) Levels of apoptosis 48 h after different combinations of treatment in SiHa cells. (D) Levels of apoptosis in MCF7 cells cannot be detected as they harbor a CASP3 mutation, treated as in (C). (E) Limiting dilution analysis for BCSCs treated as in (C). (F) Flow cytometer plots presenting results of nicoletti assay in SiHa cells. All experiment were performed at least three times, independently and error bars represent SD, significance is indicated with horizontal lines (*P < 0.05, **P < 0.01, ***P < 0.001).