Complete Genome Sequences of Six Copper-Resistant Xanthomonas Strains Causing Bacterial Spot of Solaneous Plants, Belonging to X. gardneri, X. euvesicatoria, and X. vesicatoria, Using Long-Read Technology

ABSTRACT

Xanthomonas vesicatoria, Xanthomonas euvesicatoria, and Xanthomonas gardneri cause bacterial spot disease. Copper has been applied since the 1920s as part of integrated management programs. The first copper-resistant strains were reported some decades later. Here, we fully sequenced six Xanthomonas strains pathogenic to tomato and/or pepper and having a copper-resistant phenotype.

GENOME ANNOUNCEMENT

Bacterial spot disease causes yield loss and impairs fruit quality in most tomato- and pepper-producing areas. The disease is caused by several distinct Xanthomonas species: X. vesicatoria, X. euvesicatoria, X. gardneri, and X. perforans, with X. perforans being recently reclassified as X. euvesicatoria (1). These phylogenetically distant species display similar symptoms and host range (2). Tomato (Lycopersicon esculentum), sweet pepper (Capsicum annuum), and chili pepper (Capsicum frutescens) are the main natural host species. The large-scale spread of the pathogens occurs through contaminated seeds, requiring strict disease management strategies to be imposed. A research effort is being made on biocontrol strategies, such as bacteriophage use, because resistant cultivars are scarce and rapidly overcome, and bacterial resistance to antimicrobial compounds is often reported. For example, widely used on pepper since the 1920s in the United States (3), a failure of copper sprays to control bacterial spot disease was first observed in 1968 (4), and the first associated genetic determinant (i.e., a transmissible plasmid) was reported more than 20 years later (2, 5).

In order to obtain a better understanding of copper resistance determinants and their spread among xanthomonads, we sequenced six tomato and pepper pathogen strains displaying a copper-resistant phenotype: X. euvesicatoria LMG930 and LH3, X. vesicatoria LMG911 and LM159, and X. gardneri JS749-3 and ICMP7383.

The long-read PacBio RSII technology was used to fully sequence the six strains, using one single-molecule real-time (SMRT) cell for each strain. Assembly of the raw reads was then performed using a SMRT Analysis HGAP version 2.3 protocol, and circularization of the contigs was done using a combination of the Minimus assembler (6) and the SMRT Analysis resequencing version 1 protocol. Assembly of transcription activator-like genes was improved using a custom version of the method previously described (7).

We obtained six closed chromosomes with sizes ranging from 4,969,893 bp to 5,313,102 bp, along with two to four closed plasmids per strain (31,328 bp to 222,061 bp) and uncircularized contigs. Average nucleotide identity (ANI) scores with type strains were used to confirm species assignation (Table 1). The six strains possessed the previously described copper resistance gene system copLAB (8) carried on a conjugative plasmid. Interestingly, the plasmid was very well conserved among five strains (dating from 1955 to 2010), while the other (LMG930) had no similarities apart from the copper resistance gene cluster. On five strains, this plasmid also comprised the cusAB-smmD, czcABCD, and arsBHCR resistance gene cluster. LH3 solely lacked arsBHCR. Evaluation of the minimum inhibitory concentrations was achieved on casitone yeast extract glycerol (CYE) medium, as previously reported (9, 10): these were zinc chloride, 16 to 32 mg/liter; copper sulfate, 128 to 256 mg/liter; sodium arsenite, 16 mg/liter (LH3), 128 to 512 mg/liter (other strains); cadmium sulfate, 6.4 to 12.8 mg/liter; and cobalt chloride, 16 to 32 mg/liter.

TABLE 1 .

Characteristics of the six sequenced Xanthomonas strains^a

Strain	Other no.	Species	Copper resistance location	Country of isolation	Yr of isolation	Host	Chromosome size (bp)	G+C content (%)	ANI score (%)b	Accession no.
LMG930		X. euvesicatoria	Plasmid	United States	1969	Pepper	5,079,107	64.7	99.98	CP018463 to CP018467
LMG911	CFBP2537	X. vesicatoria	Plasmid	New Zealand	1955	Tomato	5,110,163	64.3	99.95	CP018725 to CP018727
LM159	Bv-5-4a (INTA)	X. vesicatoria	Plasmid	Argentina	1987	Pepper	5,086,726	64.2	99.88	CP018468 to CP018471
LH3	Xv5 (FAREI), CFBP7993	X. euvesicatoriac	Plasmid	Mauritius	2010	Tomato	4,969,893	64.9	98.68 (99.98)	CP018472 to CP018476
JS749-3		X. gardneri	Plasmid	Réunion	1997	Tomato	5,158,913	63.7	99.91	CP018728 to CP018730
ICMP7383	CFBP7999	X. gardneri	Plasmid	New Zealand	1980	Tomato	5,313,102	63.5	98.29	CP018731 to CP018734

^aLMG strains are from the Belgian Coordinated Collections of Microorganisms (BCCM/LMG), University of Ghent, Belgium. ICMP, International Collection of Microorganisms from Plants, Landcare Research, Auckland, New Zealand; INTA, Instituto Nacional de Tecnología Agropecuaria; FAREI, Food and Agricultural Research and Extension Institute; CFBP, Collection Française de Bactéries Associées aux Plantes.

^bANI scores were calculated using type strains of each species: LMG27970 for X. euvesicatoria; ATCC 35937 for X. vesicatoria; and ATCC 19865 for X. gardneri. Value in parentheses is for the type strain of X. perforans (CFBP7293).

^cSynonym of X. perforans.

The very high similarity of the plasmids hosting the copper resistance adaptive trait among four species pathogenic to solaneous plants suggests pervasive events of horizontal gene transfer at the niche level.

Accession number(s).

The six closed genome sequences have been deposited at GenBank. The genome accession numbers are shown in Table 1.