The ompU Paralogue vca1008 Is Required for Virulence of Vibrio cholerae

Volume 186, no. 15, p. 5167-5171, 2004. Due to an illegitimate recombination event, the triple porin mutant (ΔompU ΔompT Δvc0972) we reported is still UmpU⁺. The correct strain has been constructed and exhibits different phenotypes than what was reported. It grows in LB broth with a similar doubling time and final cell density as the parent strain, GOA1264 (OmpU⁺ OmpT⁺ VC0972⁺). However, it is outcompeted by GOA1264 ∼200-fold in a competition in LB broth at 37°C after 16 generations and is outcompeted 3-fold in competition in infant mice. In addition, the outer membrane protein profile of the triple mutant is indistinguishable from that of the ΔompU single and Δvca1008 ΔompU double mutants.