Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1990 Jan;87(1):274–277. doi: 10.1073/pnas.87.1.274

Production of abiotic receptors by molecular imprinting of proteins.

L Braco 1, K Dabulis 1, A M Klibanov 1
PMCID: PMC53245  PMID: 2153288

Abstract

When a protein is dissolved in a concentrated aqueous solution of a multifunctional organic compound, freeze-dried, and washed with an anhydrous organic solvent to remove the ligand, the resultant "imprinted" protein preparation binds up to 30-fold more of the template compound in anhydrous solvents than the nonimprinted protein in the same solvent (and both proteins in water). These artificial receptors exhibit marked ligand selectivity as well as stability in anhydrous media. This phenomenon of molecular imprinting, demonstrated for several unrelated proteins and ligands, may be helpful in the development of unique bioadsorbents and, potentially, new biocatalysts.

Full text

PDF
274

Images in this article

276Image
on p.276

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Breslow R. Artificial enzymes. Science. 1982 Nov 5;218(4572):532–537. doi: 10.1126/science.7123255. [DOI] [PubMed] [Google Scholar]
  2. Cram D. J. The design of molecular hosts, guests, and their complexes. Science. 1988 May 6;240(4853):760–767. [PubMed] [Google Scholar]
  3. Karplus M., McCammon J. A. Dynamics of proteins: elements and function. Annu Rev Biochem. 1983;52:263–300. doi: 10.1146/annurev.bi.52.070183.001403. [DOI] [PubMed] [Google Scholar]
  4. Klibanov A. M. Enzymatic catalysis in anhydrous organic solvents. Trends Biochem Sci. 1989 Apr;14(4):141–144. doi: 10.1016/0968-0004(89)90146-1. [DOI] [PubMed] [Google Scholar]
  5. Kuntz I. D., Jr, Kauzmann W. Hydration of proteins and polypeptides. Adv Protein Chem. 1974;28:239–345. doi: 10.1016/s0065-3233(08)60232-6. [DOI] [PubMed] [Google Scholar]
  6. Napper A. D., Benkovic S. J., Tramontano A., Lerner R. A. A stereospecific cyclization catalyzed by an antibody. Science. 1987 Aug 28;237(4818):1041–1043. doi: 10.1126/science.3616626. [DOI] [PubMed] [Google Scholar]
  7. Rebek J., Jr Model studies in molecular recognition. Science. 1987 Mar 20;235(4795):1478–1484. doi: 10.1126/science.3823899. [DOI] [PubMed] [Google Scholar]
  8. Russell A. J., Klibanov A. M. Inhibitor-induced enzyme activation in organic solvents. J Biol Chem. 1988 Aug 25;263(24):11624–11626. [PubMed] [Google Scholar]
  9. Schultz P. G. The interplay between chemistry and biology in the design of enzymatic catalysts. Science. 1988 Apr 22;240(4851):426–433. doi: 10.1126/science.2833815. [DOI] [PubMed] [Google Scholar]
  10. Weetall H. H. Covalent coupling methods for inorganic support materials. Methods Enzymol. 1976;44:134–148. doi: 10.1016/s0076-6879(76)44012-0. [DOI] [PubMed] [Google Scholar]
  11. Zaks A., Klibanov A. M. Enzymatic catalysis in nonaqueous solvents. J Biol Chem. 1988 Mar 5;263(7):3194–3201. [PubMed] [Google Scholar]
  12. Zaks A., Klibanov A. M. Enzymatic catalysis in organic media at 100 degrees C. Science. 1984 Jun 15;224(4654):1249–1251. doi: 10.1126/science.6729453. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES