Fig. 2.
In vitro grown oocytes recovered from follicles (A, 200X) cultured in isotonic growth medium for 15 d or (B, 200X) pre-incubated in the same medium at 350 mOsm for 24 h before the same isotonic culture for 15 d. Morphology of oocytes from the latter culture condition (C, 200X) after in vitro maturation (D, 400X). The arrow indicates first polar body. Bars represent 100 µm.