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. 2016 Aug 20;7(39):63215–63225. doi: 10.18632/oncotarget.11438

Figure 2. FAM65B expression disrupts the formation of the mitotic spindle, resulting in mitotic failure.

Figure 2

(A) The cell cycle of HeLa cells transfected with GFP alone or FAM65B fused with GFP expression vectors was analyzed by flow cytometry two days after transfection. Results are representative of three experiments. The time required for each HeLa cell co-transfected with Histone H2B-mCherry and GFP (n = 22) or FAM65B-GFP (n = 27) expressing vectors to complete a division (B and D) or to align chromosomes (C) was evaluated by time-lapse microscopy. Statistical analysis: Paired two-tailed Student's t-test. (E) HeLa cells were co-transfected with α-tubulin-GFP and m-Cherry (n = 14) or FAM65B-mCherry (n = 20), and their capacity to form a mitotic spindle was followed by time-lapse microscopy.