Skip to main content
. 2016 Aug 26;7(39):63679–63689. doi: 10.18632/oncotarget.11626

Figure 5. Leucine deprivation decreases FASN expression via GCN2/SREBP1C signaling.

Figure 5

(A and C) MDA-MB-231 or MCF-7 cells were incubated in control (+leu) or leucine-deficient (−leu) medium for 48 h, followed by examination of the corresponding mRNA in A and proteins in C. (B) Cells expressing the Fasn promoter vector were incubated in control (+leu) or leucine-deficient (−leu) medium for 48 h, followed by examination of luciferase activity. (D) Cells with (+GCN2 RNAi) or without (−GCN2 RNAi) GCN2 knockdown were incubated in control (+leu) or leucine-deficient (−leu) medium for 48 h, followed by examination of the corresponding proteins. Statistical significance was calculated using the two-tailed Student t test for the effects of (−) leu vs. the control treatment (*p < 0.05) in A–C, or using the one-way ANOVA followed by the Student- Newman-Keuls (SNK) test for the effects of (−) leu vs. the control treatment (*p < 0.05) in D.