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. 2016 Aug 31;7(39):64053–64070. doi: 10.18632/oncotarget.11771

Figure 1. Optimum conditions for the fluorescence intensity measurements of pooled serum samples.

Figure 1

(A) The fluorescence intensity ratios of PHC to LC, CH and NC correspond to pooled serum volumes at 37°C (T37). (B) The fluorescence intensity ratios of PHC to LC, CH and NC correspond to detection temperatures for 3 μL (S3) and 15 μL (S15) of pooled serum. (C, D) The fluorescence intensity ratios of PHC to LC, CH and NC correspond to EvaGreen volumes for 3 μL (S3) and 15 μL (S15) of pooled serum at 8°C (T8) and 37°C (T37). (E, F) The fluorescence intensity ratios of PHC to LC, CH and NC correspond to the cycle numbers (incubation time) for 3 μL of pooled serum at 8°C (S3T8) and 15 μL of pooled serum at 37°C (S15T37) in the absence of EvaGreen and for 3 μL of pooled serum at 8°C (S3T8E) and 15 μL of pooled serum at 37°C (S15T37E) in the presence of EvaGreen. PHC: primary hepatic carcinoma; LC: liver cirrhosis; CH: chronic hepatitis; NC: normal control.