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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Virology. 2017 Jan 23;503:52–61. doi: 10.1016/j.virol.2017.01.009

Fig 3.

Fig 3

Comparison of 2.1-kb RNA transcription and SPII promoter activities between genotype A and genotype D clones. (A) Northern blot analysis of HBV RNAs from Huh7 cells transiently transfected with 0.6mer constructs of 6 genotype A clones and 7 genotype D clones. Positions of 2.1-kb and 0.7-kb HBV RNAs are indicated. The same blot was hybridized with a GAPDH probe for loading control. (B) Schematic representation of the envelope gene, the SPI promoter driving 2.4-kb RNA, the SPII promoter driving the 2.1-kb RNA, and the three envelope proteins translated from these RNA species. (C and D) Shown on top is the schematic representation of the firefly luciferase reporter construct driven by the SPI or SPII promoter. Due to a 6-bp insertion in genotype A genomes and a 33-bp deletion in genotype D genomes, numbering of SPI and SPII promoters is different for the two genotypes despite identical sizes. Shown at the bottom are results of firefly luciferase/vanilla luciferase ratio from Huh7 cells transiently transfected with such a reporter construct from 3 genotype A clones (plain) and 3 genotype D clones (bold). A total of 4 transfection experiments were performed.