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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Virology. 2017 Jan 23;503:52–61. doi: 10.1016/j.virol.2017.01.009

Fig 8.

Fig 8

Impact of exchanging the S region on the ratio of extracellular HBsAg/intracellular HBsAg from 0.7mer expression construct. The S region was exchanged between three pairs of genotype A - genotype D clones: 5.4 (A) and 1.2 (D), 6.5 (A) and 20.11 (D), as well as 9.4 (A) and 30.4 (D). (A) Northern blot analysis of the 2.4-kb/2.1-kb RNAs. (B) Western blot analysis of intracellular envelope proteins. (C and D) ELISA for intracellular HBsAg (1:400 dilution) and extracellular HBsAg (1:200 dilution). (E) Ratio of extracellular HBsAg/intracellular HBsAg. Results were averaged from 6 transfection experiments.