LETTER
Macrolide antibiotics are the first-line treatment for Mycoplasma genitalium infections; however, macrolide resistance has increased up to 40% in several countries (1–3). Consequently, the 2016 European guideline on M. genitalium infections has recommended complementing the molecular detection of M. genitalium with an assay capable of detecting macrolide resistance-associated mutations (4).
We aimed to evaluate the clinical performance of the CE-marked ResistancePlus MG kit (SpeeDx, Australia), which utilizes PlexZyme/PlexPrime technology (5) for the detection of M. genitalium (MgPa adhesin gene) and the five predominant 23S rRNA macrolide resistance-associated mutations (A2058G, A2059G, A2058C, A2059C, and A2058T [Escherichia coli numbering]). This was compared to in-house assays using real-time quantitative PCR (qPCR) to detect M. genitalium and real-time PCR and melting curve analysis to detect the macrolide resistance-associated mutations (“macrolide resistance qPCR”) (6).
A total of 206 male and female urogenital specimens previously analyzed using an in-house method (7) and conserved at −80°C (94 M. genitalium-positive and 112 M. genitalium-negative specimens) were retrospectively and systematically selected from samples collected in 2014 to 2015 at the Bordeaux University Hospital (France). A 5-μl volume of the internal control provided in the kit was spiked into 200 μl of specimen before extraction, which was performed using a MagNA Pure 96 instrument (Roche Diagnostics). The ResistancePlus MG and in-house assays were performed using the Cobas z480 analyzer of the Cobas 4800 platform (Roche Diagnostics), according to the instructions of the manufacturers. An additional M. genitalium detection assay, using the CE-marked S-DiaMGTV kit (Diagenode, Belgium) (8, 9), was performed such that any two of the possible three comparator results would define infection status. Pyrosequencing (1) was performed for four specimens that could not be amplified using the in-house macrolide resistance qPCR. The reference method for determining the presence of macrolide resistance-associated mutations was 23S rRNA sequencing (6). The ResistancePlus MG kit provided did not discern between absence of mutation and absence of 23S rRNA gene amplification. A 23S rRNA amplification control would be beneficial in a future version of the assay.
For M. genitalium detection, there was a 0.48% (1/206) rate of invalid results due to internal control failure. The concordance between the ResistancePlus MG kit result and the M. genitalium patient status was 99.5%, with a kappa value of 0.98. The clinical sensitivity and specificity were 98.9% and 100%, respectively (Table 1).
TABLE 1.
Clinical performance characteristics of the ResistancePlus MG kit in comparison to M. genitalium infection status
| M. genitalium infection status | ResistancePlus MG kit characteristic(s)a |
|||||
|---|---|---|---|---|---|---|
| No. of positive results | No. of negative results | % sensitivity (95% CI) | % specificity (95% CI) | PPV (95% CI) | NPV (95% CI) | |
| Positive | 94 | 1 | 98.9 (94.3–99.8) | 100 (96.6–100) | 100 (96.1–100) | 99.1 (95.1–99.8) |
| Negative | 0 | 110 | ||||
CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value.
According to the M. genitalium-positive patient status, 93 M. genitalium-positive specimens were retained for the macrolide resistance evaluation (Table 2). Among them, four specimens presented discrepant results between the macrolide resistance qPCR and the ResistancePlus MG tests. Three specimens were called as mutants by the ResistancePlus MG kit but not by the macrolide resistance qPCR. Sequencing of the 23S rRNA confirmed the absence of mutations. For the remaining specimen, sequencing identified an A2062T mutation, which is not included in the ResistancePlus MG kit. Overall, the concordance between the ResistancePlus MG kit and the macrolide resistance status was 95.6%, with a kappa value of 0.88. As previously reported (5), the clinical sensitivity and specificity of the ResistancePlus MG kit were good (95.4% and 95.8%, respectively).
TABLE 2.
Clinical performance characteristics of the ResistancePlus MG kit in comparison to macrolide resistance status
| Macrolide resistance status | ResistancePlus MG kit characteristic(s)a |
|||||
|---|---|---|---|---|---|---|
| No. of results with presence of mutation | No. of results with no mutation | % sensitivity (95% CI) | % specificity (95% CI) | PPV (95% CI) | NPV (95% CI) | |
| Presence of mutation | 21 | 1 | 95.4 (78.2–99.2) | 95.8 (88.3–98.5) | 87.5 (69.0–95.7) | 98.6 (92.2–99.7) |
| No mutation | 3 | 68 | ||||
CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value.
In conclusion, the ResistancePlus MG kit is a rapid and reliable method to simultaneously detect M. genitalium and determine macrolide resistance in clinical specimens.
ACKNOWLEDGMENTS
We thank J. S. Jensen for performing pyrosequencing assay on specimens that were not amplified using the in-house resistance PCR.
This evaluation was supported by a research grant from SpeeDx, Australia. The funders had no role in data collection and interpretation.
REFERENCES
- 1.Salado-Rasmussen K, Jensen JS. 2014. Mycoplasma genitalium testing pattern and macrolide resistance: a Danish nationwide retrospective survey. Clin Infect Dis 59:24–30. doi: 10.1093/cid/ciu217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Tagg KA, Jeoffreys NJ, Couldwell DL, Donald JA, Gilbert GL. 2013. Fluoroquinolone and macrolide resistance-associated mutations in Mycoplasma genitalium. J Clin Microbiol 51:2245–2249. doi: 10.1128/JCM.00495-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Getman D, Jiang A, O'Donnell M, Cohen S. 2016. Mycoplasma genitalium prevalence, coinfection, and macrolide antibiotic resistance frequency in a multicenter clinical study cohort in the United States. J Clin Microbiol 54:2278–2283. doi: 10.1128/JCM.01053-16. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Jensen JS, Cusini M, Gomberg M, Moi H. 2016. 2016 European guideline on Mycoplasma genitalium infections. J Eur Acad Dermatol Venereol 30:1650–1656. doi: 10.1111/jdv.13849. [DOI] [PubMed] [Google Scholar]
- 5.Tabrizi SN, Tan LY, Walker S, Twin J, Poljak M, Bradshaw CS, Fairley CK, Bissessor M, Mokany E, Todd AV, Garland SM. 2016. Multiplex assay for simultaneous detection of Mycoplasma genitalium and macrolide resistance using PlexZyme and PlexPrime technology. PLoS One 11:e0156740. doi: 10.1371/journal.pone.0156740. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.Touati A, Peuchant O, Jensen JS, Bébéar C, Pereyre S. 2014. Direct detection of macrolide resistance in Mycoplasma genitalium isolates from clinical specimens from France by use of real-time PCR and melting curve analysis. J Clin Microbiol 52:1549–1555. doi: 10.1128/JCM.03318-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Jensen JS, Bjornelius E, Dohn B, Lidbrink P. 2004. Use of TaqMan 5′ nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic. J Clin Microbiol 42:683–692. doi: 10.1128/JCM.42.2.683-692.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 8.Le Roy C, Pereyre S, Bébéar C. 2014. Evaluation of two commercial real-time PCR assays for detection of Mycoplasma genitalium in urogenital specimens. J Clin Microbiol 52:971–973. doi: 10.1128/JCM.02567-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Pereyre S, Laurier Nadalié C, Bébéar C, Investigator group. 9 November 2016. Mycoplasma genitalium and Trichomonas vaginalis in France: a point prevalence study in people screened for sexually transmitted diseases. Clin Microbiol Infect doi: 10.1016/j.cmi.2016.10.028. [DOI] [PubMed] [Google Scholar]