Accuracy of molecular diagnostics in pemphigus and bullous pemphigoid: comparison of commercial and modified mosaic indirect immunofluorescence tests as well as enzyme-linked immunosorbent assays

Abstract

Introduction

Pemphigus and bullous pemphigoid (BP) are identified by autoantibodies (abs) against desmoglein 1, 3 (DSG1/3) and BP180/BP230, respectively. A novel mosaic to indirect immunofluorescence (IIF) using purified BP180 recombinant proteins spotted on slide and transfected cells expressing BP230, DSG1, DSG3 is available. The commercial (IgG detection) and modified (IgG4 detection) mosaic for indirect immunofluorescence (IIFc – IIF commercial, IIFm – IIF modified) and IgG ELISAs were evaluated in pemphigus and bullous pemphigoid (BP) molecular diagnostics.

Aim

To compare diagnostic accuracy of commercial (IgG detection) and modified (IgG4 detection) mosaic IIF assay and to examine the diagnostic value of ELISAs in relation to mosaic IIF in routine laboratory diagnostics of pemphigus and BP.

Material and methods

Sera from 37 BP and 19 pemphigus patients were studied. Associations between tests were assessed using Fisher’s exact test.

Results

There are associations between the positive/negative samples detected by IIFc with desmoglein1 (DSG1)/desmoglein3 (DSG3)/BP230 transfected cells and ELISAs and no association between anti-BP180 IgG detection by IIFc and ELISA. IIFm with DSG1 and DSG3 showed both 100% sensitivity and 100% and 78% specificity, respectively, and 100% and 83% positive predictive value in relation to IIFc. IIFm with BP230 had 87% specificity, 55% sensitivity, whereas IIFm with BP180 had a 100% sensitivity and 13% specificity in relation to IIFc.

Conclusions

The IIFc with DSG1/DSG3/BP230 transfected cells, excluding BP180 spots, is an alternative method to ELISA in pemphigus/BP diagnostics. IgG4 antibodies, both pathogenically and diagnostically important, are inconsistently detectable with IIFm.

Introduction

Autoimmune blistering dermatoses (ABD) are immunologically characterized by the pathological autoimmune responses directed against cell-cell or cell-matrix adhesion molecules [1]. More common ABD entities, pemphigus and bullous pemphigoid (BP), are identified by circulating and tissue-bound autoantibodies (abs) against the desmosomal cadherins (mainly desmoglein 1 and 3 – DSG1/3) and dermal-epidermal junction components (BP180 and BP230), respectively. Autoimmunity in these dermatoses is associated with stimulation of Th1/Th2 cells responsible for pathogenic abs production by B cells and these patients may contain Th1-regulated IgG1 and Th2-regulated IgG4 abs directed against DSG1/DSG3 or BP180/BP230, respectively [2]. Detected abs usually belong to the IgG class, however the significance of IgG1 and IgG4 subclasses seems to be still equivocal.

Recently, the complex and heterogeneous pathogenesis of ABD has been revealed. Clinical heterogeneity determines that ABD can mimic a variety of clinical conditions with unusual variants of disease, including non-bullous clinical manifestations [3]. In light of this, isotype/class switching, autoantibody shift, enhanced immunogenicity of antigens by specific triggering factors (including drugs) [4] through the exposition of sequestered antigens to the immune system as well as conformational changes may underline the molecular heterogeneity of ABD. Thus, clinical and molecular heterogeneity of ABD poses a surprisingly great diagnostic challenge to the dermatologists making their diagnostic work a complex multistep process.

Nowadays, the diagnosis of ABD is generally based on a combination of various criteria: clinical features, histology and immunological tests, notably direct and indirect immunofluorescence (DIF and IIF, respectively) and enzyme linked immunosorbent assay (ELISA) [5]. The precise identification of the target antigen is crucial for patient management. Several assays for the serological detection of anti-DSG1/DSG3/BP180/BP230 abs have been developed, however no generally accepted gold standard assay exists, and thus a working set for as definitive diagnosis as possible is urgently needed.

Recently, novel techniques for serological ABD diagnosis, such as biochip mosaic-based and bioplex-based techniques, have been discussed [6–9]. A novel IIF microscopy approach using purified BP180 recombinant proteins spotted on slide and transfected cells expressing BP230, DSG1, DSG3 is available.

Here, we describe a modified BIOCHIP mosaic-based indirect immunofluorescence (modified indirect immunofluorescence – IIFm) technique for the determination of anti-DSG1, anti-DSG3, anti-BP180 and anti-BP230 IgG4 abs based on recombinant antigenic substrates and transfected cells in relation to commercial BIOCHIP mosaic-based IIF (commercial indirect immunofluorescence – IIFc) for the determination IgG abs. In this study we investigated the sensitivity, specificity as well as positive and negative predictive values of this innovative immunoassay in order to evaluate its diagnostic use for the laboratory diagnosis of ABD compared with ELISAs.

Aim

The aim of this study was to compare the diagnostic performance of original and modified multiparametric IIF assay for ABD serology. We attempted to modify original mosaic-based IIF for IgG to pemphigus and BP antigens (DSG1/DSG3/BP180/BP230) in order to enable assessment of IgG4 abs to those antigens.

The second aim was to examine the diagnostic value of commercial ELISAs in relation to mosaic-based IIF with transfected cells in routine laboratory diagnostics of ABD in a setting in Poland.

Material and methods

This study was approved by the local Ethical Committee of the Poznan University of Medical Sciences in Poland and informed written consent was obtained from each individual.

Patients and serum samples

Altogether, 56 patients with ABD before initiation of treatments were investigated. The examined group involved 19 pemphigus patients (pemphigus vulgaris and pemphigus foliaceus) and 37 BP patients. Patients in the examined groups met following criteria: (i) clinical features – flaccid blisters and erosions on the skin and mucous membranes in pemphigus; tense cutaneous blisters with no or transient involvement of mucosal surfaces and sparing head and neck in BP, (ii) typical immunoglobulin deposits detected with DIF of perilesional skin, (iii) molecular characterization of antigens. The diagnosis of BP was made in patients having IgG/IgG4 and/or IgG1 non-U-pattern deposits along the dermal-epidermal junction in DIF, diagnosis was corroborated with positive BP180 and/or BP230 IgG ELISA in serum samples. Other subepidermal autoimmune blistering dermatoses could be excluded based on these data. The diagnosis of pemphigus was made in patients having IgG/IgG4/IgG1 fish-net like or dewdrops on spider web patterns in DIF, diagnosis was corroborated with DSG1/DSG3 IgG ELISA in serum samples. The diagnosis of ABD was supported by histology.

Patients were recruited during the period of October 2013 through February 2015 at the Autoimmune Blistering Dermatoses Section, Department of Dermatology, Poznan University of Medical Sciences, Poland. The serum used in the serological tests was taken at the time of hospital admission/ambulatory care. Five ml of blood serum were obtained from each participant. The samples were centrifuged for 10 min at 3500 rpm. Thereafter, they were stored at –20°C until performing ELISA and BIOCHIP mosaic IIF.

ELISA

The specific circulating serum abs were detected with commercially available ELISAs. ELISAs were performed using the Euroimmun (Luebeck, Germany) ELISA kits, utilizing recombinant protein DSG1, DSG3, BP180, BP230, with the manufacturer’s cut-off value of 20 RU/ml. Anti-BP180-NC16A-4X ELISA includes four copies of domain NC16A fused to a polyhistidine tag to enhance protein expression. Anti-BP230-CF ELISA contains an amplified fragment of C-terminal globular domain. Anti-DSG1 IgG and anti-DSG3 IgG was measured with an ELISA assay utilizing recombinant proteins DSG1 and DSG3, consisting of the extracellular domain of DSG1 and DSG3, respectively (5 subdomains). All measurements were made in the ELISA plate reader (Asys Expert 96) equipped with Microwin 2000 software by a single operator following the manufacturer’s instructions.

BIOCHIP mosaic IIF

BIOCHIP-mosaic based IIF (EUROPLUS Dermatology Mosaic, Euroimmun, Luebeck, Germany) consists of an array of six different diagnostic substrates including monkey esophagus, primate salt-split skin, dots of tetrameric BP180-NC16A as well as DSG1 and DSG3 extracellular and transmembrane domains (E + TM) and BP230 C-terminal domain expressed in HEK293 (human embryonic kidney) cells. The reaction was performed following the manufacturer’s instructions. The original BIOCHIP with the assessment of anti-DSG1/DSG3/BP180/BP230 IgG was modified to enable assessment of IgG4 abs to those antigens (Figures 1 A–D, Figures 2 A–D). The IIFm was provided by replacing the standard fluorescein-conjugated anti-human IgG (Euroimmun, Germany) by a mouse monoclonal anti-human fluorescein-conjugated IgG4 secondary antibody (1 : 100 dilution; Sigma, USA). The procedure of IIF consisted of two consecutive incubation steps. Firstly, diluted (1 : 10) serum samples were incubated for 30 min at room temperature (RT). In the second 30 min at RT the attached autoantibodies are stained with fluorescein-labeled anti-human IgG, IgG4 antibodies.

Figure 1.

Indirect immunofluorescence mosaic for autoimmune blistering dermatoses in a 78-year-old woman with bullous pemphigoid (DIF of perilesional skin revealed linear IgG1, IgG4, C3 deposits along the dermal-epidermal junction; ELISA serum anti-BP180-NC16A-4X IgG 172.809 RU/ml, anti-BP230-C IgG above 200 RU/ml, cut-off 20 RU/ml): positive reaction with IgG (A), IgG4 (B) autoantibodies against BP230-C expressed in transfected HEK293 cells and positive reaction with IgG (C), but negative reaction with IgG4 (D), autoantibodies against the tetrameric BP180 NC16A spots (antigen dots)

Figure 2.

Indirect immunofluorescence mosaic for autoimmune blistering dermatoses in a 71-year-old woman with mucocutaneous pemphigus vulgaris (DIF of perilesional skin revealed pemphigus-type IgG1, IgG4, C3 deposits in the epidermis; ELISA serum anti-DSG1 IgG 91. 593 RU/ml, anti-DSG3 IgG above 200 RU/ml, cut-off 20 RU/ml): positive reaction with IgG (A), IgG4 (B) autoantibodies against extracellular and transmembrane domains of DSG1 expressed in transfected HEK293 cells and positive reaction with IgG (C), IgG4 (D) autoantibodies to extracellular and transmembrane domains of DSG3 expressed in transfected HEK293

All slides were evaluated simultaneously by two observers using a microscope (BX40, Olympus, Japan) with a fluorescent adapter.

Statistical analysis

The accuracy of modified mosaic IIF and ELISA was evaluated by calculating diagnostic sensitivity, diagnostic specificity as well as positive and negative predictive values in relation to the original/commercial mosaic IIF using the MedCalc Software 2015 (Ostend, Belgium; www.medcalc.org). Associations in results between tests were assessed using Fisher’s exact test. A p < 0.05 was arbitrarily considered statistically significant.

Results

Comparison of diagnostic accuracy between modified (IgG4) mosaic IIF and original (IgG) mosaic IIF

The diagnostic sensitivity and specificity as well as positive and negative predictive values of modified (IgG4) in comparison with standard (IgG) IIF are shown in Table 1.

Table 1.

Calculation of the diagnostic sensitivity, specificity and predictive values of multiparametric IIF (modified versus original)

Diagnostic accuracy of modified mosaic IIF in relation to original mosaic IIF
Modified (IgG4) versus original (IgG) IIF	Subjects (n)	Number of positive results		Number of negative results		Sensitivity (%)	Specificity (%)	Positive predictive value (PPV) (%)	Negative predictive value (NPV) (%)
		IgG	IgG4	IgG	IgG4
DSG1	19	13	13	6	6	100	100	100	100
DSG3	19	12	10	7	9	100	78	83	100
BP180	37	33	7	4	30	100	13	21	100
BP230	37	9	6	28	31	55	78	62	84

IIF – indirect immunofluorescence, DSG1 – desmoglein 1, DSG3 – desmoglein 3, BP180 – bullous pemphigoid antigen 180, BP230 – bullous pemphigoid antigen 230, n – number of patients.

Comparison of diagnostic accuracy between original (IgG) mosaic IIF and ELISA

The diagnostic sensitivity and specificity as well as positive and negative predictive values of original mosaic (IgG) IIF in relation to ELISA are shown in Table 2.

Table 2.

Calculation of the diagnostic sensitivity, specificity and predictive values of original multiparametric IIF in relation to ELISA

Diagnostic accuracy of original mosaic IIF in relation to ELISA
Original mosaic IIF (IgG) versus ELISA (IgG)	Subjects (n)	Results of ELISA		Sensitivity (%)	Specificity (%)	Positive predictive value (PPV) (%)	Negative predictive value (NPV) (%)
		Number of positive results	Number of negative results
DSG1	19	12	7	100	85	92	100
DSG3	19	13	6	100	85	92	100
BP180	37	36	1	91	100	100	25
BP230	33	9	24	55	87	62	84

IIF – indirect immunofluorescence, DSG1 – desmoglein 1, DSG3 – desmoglein 3, BP180 – bullous pemphigoid antigen 180, BP230 – bullous pemphigoid antigen 230, n – number of patients.

Associations between modified (IgG4), original (IgG) mosaic IIF and ELISA

There is an association between the values of positive/negative samples detected by original/commercial IIF with DSG1/DSG3/BP230 transfected cells and ELISAs (p < 0.05). There is no association between anti-BP180 IgG detection in BP patients by IIF and ELISA (p = 0.1081).

There is an association between the values of positive/negative samples detected by modified (IgG4) mosaic IIF with DSG1/DSG3/BP230 transfected cells and original/commercial (IgG) mosaic IIF (p < 0.05) (Figures 1 A, B, Figures 2 A–D).

There is no association between anti-BP180 IgG4 detected by modified IIF and anti-BP180 IgG detected by original/commercial mosaic IIF (p = 0.5894) (Figures 1 C, D).

Discussion

To date, no previous study has stringently compared the two mosaic IIF assays (commercially available and innovative modification to characterize the IgG4 reactivity in sera considering that the production of abs in pemphigus and BP is polyclonal) in terms of their use in the diagnosis of pemphigus and BP in a defined population. Moreover, in extension of previous findings, our study investigated mosaic IIF with DSG1/DSG3/BP230 transfected cells and BP180 NC16A spots as an alternative way to ELISAs in management of pemphigus and BP patients.

Our results indicated that transfected cells may be useful, with the diagnostic accuracy similar to ELISAs, in laboratory diagnostics of pemphigus and BP supporting previous investigations [6–9]. A novel finding of our study was the infrequent detection of BP180-reactive IgG4 on BP180 NC16A spots with IIF. However, it should be noted that data obtained by various authors depended on the applied antigen recombinants, the selected patients and the methodology used.

Our previous observations with the use of DIF and IIF confirm that circulating abs in pemphigus and BP belong predominantly to the IgG4 isotype [2, 10]. However, the molecular specificity of detected abs was not documented there. Thus, here the molecular characterization of antigens in relation to IgG and IgG4 abs was also investigated.

The diagnostic accuracy of the transfected cells with DSG1 and DSG3 detecting IgG4 compared favourably with that of the standard transfected cells detecting IgG abs. Modified mosaic IIF with DSG1 and DSG3 showed a high sensitivity (100%), specificity (100% and 78%, respectively) and positive predictive value (100% and 83%, respectively) suggesting that the use of the modified mosaic IIF may improve the diagnosis of pemphigus in health facilities. Our data are consistent with other researchers showing the preferential detection of anti-DSG reactive IgG4 in active pemphigus vulgaris (PV) [11]. Thus, probably the use of transfected cells with DSGs might have facilitated their detection. Previous findings indicated that IgG4 subclass is probably implicated in the pathogenesis of pemphigus foliaceus (PF) and passive transfer of patients IgG4 abs has been shown to produce disease in neonatal mice [12, 13]. Interestingly, some reports demonstrated that the anti-DSG1 abs in PV are pathogenic and have IgG4 subclass specificity [14].

In case of BP patients, our results may confirm the thesis that Th2-regulated abs preferentially bind to BP230 in BP patients. The diagnostic accuracy of the transfected cells with BP230 detecting IgG4 had a good specificity (87%) but a poor sensitivity (55%) compared with that of the standard transfected cells detecting IgG abs. Interestingly, BP180 NC16A spots revealed a good sensitivity for IgG and IgG4 detection but at the expense of specificity. BP180 NC16A spots showed a high sensitivity (100%) but very low specificity (13%) and positive predictive value (21%). These findings confirm the previous study indicating Th-2 response as preferentially directed against BP230 [15]. Results obtained by Ghohestani et al. [15] using different antigenic sources showed IgE abs as directed exclusively against BP230, and not against BP180 or any other epidermal or dermal antigens. It is known that IgG4 and IgE production is regulated concordantly and requires the stimulation of Th2 cells [16]. Probably, IgG and IgG4 from BP sera were directed against the same epitope on the C-terminal end of the protein suggesting immunoglobulin class switching from IgG to IgG4. Here, the detection of anti-BP230 IgG4 abs strengthens the link between autoimmune pemphigoid and immune response involving Th2 cells.

Remarkably, in contrast to BP230, BP180 is not the target of IgG4 in our study. The lack of IgG4 reactivity to BP180 is not due to loss of antigen immunoreactivity, as it was clearly labeled by IgG abs from the same serum samples. Moreover, in some sera containing IgG against both BP230 and BP180, only IgG4 against BP230 was detected. Thus, the possibility of technical differences (various methods of BP230 and BP180 recombination) in the detection of IgG4 abs should be taken into consideration.

Our observations are contradictory with Döpp et al. [17] who analyzed the IgG subclass distribution to full-length BP180 NC16A and recombinant fragments of this domain with the use of ELISA and IB and showed that IgG4 and IgE are the major isotypes of immunoglobulin targeting BP180 NC16A. Our finding that anti-BP180 IgG4 is a nonpredominant subclass is also in contrast with Modre et al. [18] who observed this in remission, but not at the early stage of the disease. Revealed discrepancies may result from different research methods used in the studies exhibiting variable sensitivity and specificity or from detection of different antigenic regions of the examined protein. It is known that the structure of an antibody is related to its function. Thus, the protein purification and immobilization may change in protein conformation (the effect on tertiary structure of the protein) as well as the antigen alternations may have an impact on binding specificity and/or biological activity. The method used here (BP180 NC16A spots) significantly decreased the protein affinity in comparison to other diagnostic techniques – probably by low antibody avidity or loss of the antigenic structure during “spots” creation/development.

Conclusions

The most appropriate choice of a diagnostic assay for molecular characterization of autoimmunity may depend on the clinical setting. Interestingly, in this respect, it has been suggested using a BP180 humanized mouse model [19] that IgG4 abs, which should be regarded as a diagnostically useful biomarker of an active Th2-mediated autoimmunity, to BP180 NC16A may be protective, which may simply reflect the fact that the stereotypically elderly BP-affected individual is still able to cope with the disease at its active stage.

Findings obtained to date seem to indicate divergent roles for IgG4, characterized as an odd [20] subclass, abs in BP and pemphigus development, what may have an impact on disease severity (idiopathic BP as such is a less serious illness than idiopathic pemphigus).

The continuation of efforts to modify the original IIF test to enable the evaluation of IgG4 abs to ABD antigens seems justified. It seems that IIF with DSG1/DSG3/BP230 transfected cells is an alternative method to ELISA in management of pemphigus/BP patients. However, for anti-BP180 IgG detection in BP patients the ELISA remains a definitive serological test. IgG4, both pathogenically and diagnostically important, are inconsistently detectable with IIFm. IIFm may help diagnosing serologically challenging cases.

Conflict of interest

The authors declare no conflict of interest.