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. 2017 Feb 9;5(6):e00967-16. doi: 10.1128/genomeA.00967-16

100K Pathogen Genome Project: 306 Listeria Draft Genome Sequences for Food Safety and Public Health

Poyin Chen 1, Nguyet Kong 1, Bihua Huang 1, Kao Thao 1,*, Whitney Ng 1,*, Dylan Bobby Storey 1,*, Narine Arabyan 1, Azarene Foutouhi 1, Soraya Foutouhi 1,*, Bart C Weimer 1,✉
PMCID: PMC5331488  PMID: 28183778

ABSTRACT

Listeria monocytogenes is a food-associated bacterium that is responsible for food-related illnesses worldwide. This is the initial public release of 306 L. monocytogenes genome sequences as part of the 100K Pathogen Genome Project. These isolates represent global genomic diversity in L. monocytogenes.

GENOME ANNOUNCEMENT

Listeria monocytogenes is a foodborne pathogen prevalent in environmental and food matrices, including soil, fruit, and deli meats (1). This organism encompasses 13 serotypes, of which serotypes 1/2a, 1/2b, and 4b are most commonly associated with disease, including gastroenteritis and abortions (2). Listeriosis accounts for less than 1% of foodborne illnesses worldwide yet carries the highest foodborne mortality rate (16%), with immunocompromised, pregnant, young, and old individuals at risk (3). When contracted during gestation, listeriosis results in miscarriage, stillbirths, or premature labor leading to infant mortality (4). Transmission is facilitated by its capability to proliferate at low temperatures and to tolerate heat, acid stress, and salt stress, necessitating L. monocytogenes-specific sanitization protocols in processing plants (5–7).

Recent years have seen a spike in multistate outbreaks of listeriosis due to food contamination, including in deli meats, dairy, hummus, cantaloupe, and stone fruit, leading to recalls of contaminated products and resulting in millions of dollars in lost revenue (3). Genomic comparison of these outbreak strains with environmental isolates via whole-genome sequencing will shed light on the genetic factors that potentiate L. monocytogenes virulence.

The 100K Pathogen Genome Project is large-scale sequencing effort focused on producing genomes from various sources worldwide that represent isolates from the environment, plants, animals, and humans. This consortium coordinates isolate submission and DNA sequencing for pathogens of relevance to animal and human health associated with zoonotic and foodborne diseases. Sequences associated with the consortium can be found at the 100K Project website (http://100kgenomes.org). We sequenced Listeria genomes from around the world and from diverse isolation sources to better understand the genomic diversity of the genus. This also serves as a reference repository of genetic information for this group of organisms.

All cultures were shipped to the Weimer laboratory (UC Davis, Davis, CA), checked for purity, frozen, grown on 1.5% brain heart infusion (BHI) agar (Difco, Franklin Lakes, NJ), and lysed (8); genomic DNA was isolated (gDNA) (9), checked for quality (10), and fragmented (11). Libraries were 250 to 500 bp (12, 13), indexed (96/lane), and sequenced (Illumina HiSeq 2000 [PE100] [BGI@UCD; Sacramento, CA]). Paired-end reads were assembled using ABySS 1.5.2 using k = 64 (14). To date, the 100K Pathogen Genome Project has produced sequences for 313 different isolates of Listeria. In this release, the 100K Project produced 401 Mb of sequence, with an average calculated sequencing depth of 135× per genome.

Accession number(s).

Sequences can be found at the 100K Project BioProject PRJNA203445 in the Sequence Read Archive (http://www.ncbi.nlm.nih.gov/sra) (Table 1).

TABLE 1 .

Summary statistics for samples identified as Listeria monocytogenes

GenBank accession no. SRA accession no. Isolate name Avg depth of sequence (×) No. of contigs Genome size (bp) Total no. of genes Total no. of CDSsa
MJSI00000000 SRR1814333 BCW_2352 53 30 3,012,101 3,034 2,965
MJSJ00000000 SRR1814334 BCW_2353 52 20 2,888,472 2,869 2,810
MJSK00000000 SRR1814335 BCW_2354 51 22 2,991,158 3,017 2,959
MJSL00000000 SRR1814336 BCW_2355 83 31 3,052,281 3,082 3,025
MJSM00000000 SRR1814337 BCW_2356 97 20 2,964,368 2,981 2,924
MJSN00000000 SRR1814338 BCW_2357 110 23 2,939,864 2,929 2,871
MJSO00000000 SRR1814339 BCW_2359 87 18 2,916,505 2,908 2,848
MJSP00000000 SRR1814340 BCW_2361 151 16 2,945,010 2,933 2,882
MJSQ00000000 SRR1814341 BCW_2362 42 29 3,002,930 3,017 2,955
MJSR00000000 SRR1814342 BCW_2363 136 28 2,993,621 3,023 2,966
MJSS00000000 SRR1814343 BCW_2364 56 39 3,061,043 3,065 3,006
MJST00000000 SRR1814344 BCW_2365 51 24 3,022,563 3,032 2,975
MJSU00000000 SRR1814345 BCW_2367 49 21 2,944,297 2,945 2,881
MJSV00000000 SRR1814346 BCW_2368 44 13 2,930,208 2,922 2,861
MJSW00000000 SRR1814347 BCW_2369 43 21 2,771,135 2,770 2,712
MJSX00000000 SRR1814348 BCW_2370 49 33 3,106,802 3,170 3,112
MJSY00000000 SRR1814349 BCW_2371 38 20 2,957,530 2,963 2,902
MJSZ00000000 SRR1814350 BCW_2372 58 14 2,827,526 2,836 2,783
MJTA00000000 SRR1814351 BCW_2373 56 15 2,995,034 3,016 2,949
MJTB00000000 SRR1814352 BCW_2374 54 18 2,966,392 3,021 2,960
MJTC00000000 SRR1814353 BCW_2375 50 22 2,923,827 2,887 2,826
MJTD00000000 SRR1814354 BCW_2376 42 21 2,813,242 2,846 2,785
MJTE00000000 SRR1814355 BCW_2378 42 35 3,123,182 3,190 3,132
MJTF00000000 SRR1814356 BCW_2379 42 31 3,039,365 3,088 3,030
MJTG00000000 SRR1814357 BCW_2380 37 22 2,918,370 2,900 2,841
MJTH00000000 SRR1814358 BCW_2381 50 22 2,971,254 2,995 2,934
MJTI00000000 SRR1814359 BCW_2382 102 22 3,020,256 3,033 2,974
MJTJ00000000 SRR1814360 BCW_2383 61 25 3,350,928 3,350 3,290
MJTK00000000 SRR1814361 BCW_2384 64 21 2,951,592 2,975 2,914
MJTL00000000 SRR1814362 BCW_2385 55 17 2,996,412 3,017 2,952
MJTM00000000 SRR1814363 BCW_2386 42 23 2,957,654 2,959 2,902
MJTN00000000 SRR1814364 BCW_2387 51 24 3,021,344 3,031 2,973
MJTO00000000 SRR1814365 BCW_2388 54 17 2,889,294 2,870 2,811
MJTP00000000 SRR1814366 BCW_2389 49 11 2,921,087 2,930 2,875
MJTQ00000000 SRR1814367 BCW_2390 165 19 2,886,917 2,864 2,810
MJTR00000000 SRR1814368 BCW_2391 100 20 3,014,266 3,023 2,969
MJTS00000000 SRR1814369 BCW_2392 57 15 2,990,427 3,012 2,954
MJTT00000000 SRR1814370 BCW_2393 92 11 2,853,604 2,834 2,778
MJTU00000000 SRR1814371 BCW_2394 82 21 3,017,853 3,030 2,971
MJTV00000000 SRR1814372 BCW_2395 117 13 2,904,985 2,873 2,821
MJTW00000000 SRR1814374 BCW_2397 95 13 2,908,687 2,916 2,860
MJTX00000000 SRR1814375 BCW_2399 112 21 3,041,356 3,023 2,973
MJTY00000000 SRR1814376 BCW_2400 85 18 2,962,811 2,957 2,898
MJTZ00000000 SRR1814377 BCW_2401 129 13 2,794,214 2,800 2,742
MJUA00000000 SRR1814378 BCW_2402 75 25 3,021,222 3,035 2,976
MJUB00000000 SRR1814379 BCW_2403 74 23 2,962,355 2,972 2,916
MJUC00000000 SRR1814380 BCW_2404 86 13 2,934,172 2,960 2,900
MJCE00000000 SRR1814381 BCW_2405 106 12 2,882,471 2,876 2,818
MJCF00000000 SRR1814382 BCW_2406 94 20 2,898,005 2,866 2,808
MJCG00000000 SRR1814383 BCW_2407 70 22 3,048,640 3,080 3,015
MJCH00000000 SRR1814384 BCW_2408 84 15 2,993,315 3,016 2,961
MJCI00000000 SRR1814385 BCW_2409 67 20 2,977,322 2,999 2,940
MJCJ00000000 SRR1814386 BCW_2411 54 9 2,861,878 2,841 2,781
MJCK00000000 SRR1814387 BCW_2412 59 35 3,129,371 3,196 3,135
MJCL00000000 SRR1814388 BCW_2413 83 27 2,977,946 2,995 2,937
MJCM00000000 SRR1814389 BCW_2414 89 14 2,925,666 2,921 2,860
MJCN00000000 SRR1814390 BCW_2415 75 19 2,941,993 2,921 2,857
MJCO00000000 SRR1814391 BCW_2416 81 18 2,967,434 2,987 2,928
MJCP00000000 SRR1814392 BCW_2417 92 16 2,890,912 2,864 2,807
MJCQ00000000 SRR1814393 BCW_2418 76 21 3,065,940 3,086 3,027
MJCR00000000 SRR1814394 BCW_2419 68 16 2,887,580 2,857 2,791
MJCS00000000 SRR1814395 BCW_2420 52 16 2,943,342 2,942 2,877
MJCT00000000 SRR1814396 BCW_2421 57 21 3,019,802 3,028 2,969
MJCU00000000 SRR1814397 BCW_2422 53 16 2,884,219 2,850 2,788
MJCV00000000 SRR1814398 BCW_2423 96 9 2,934,518 2,923 2,859
MJCW00000000 SRR1814399 BCW_2424 114 16 2,994,257 3,009 2,947
MJCX00000000 SRR1814400 BCW_2425 131 27 2,946,581 2,938 2,872
MJCY00000000 SRR1814455 BCW_2624 92 17 2,936,653 2,909 2,854
MJCZ00000000 SRR1815434 BCW_2984 148 19 2,987,575 3,011 2,953
MJDA00000000 SRR1815435 BCW_2985 134 18 2,989,169 3,010 2,949
MJDB00000000 SRR1815436 BCW_2986 66 14 2,946,854 2,945 2,878
MJDC00000000 SRR1815437 BCW_2987 83 14 2,946,854 2,945 2,878
MJDD00000000 SRR1815438 BCW_2988 89 15 2,941,698 2,940 2,876
MJDE00000000 SRR1815439 BCW_2989 83 24 2,496,910 2,952 2,889
MJDF00000000 SRR1815440 BCW_2990 68 15 2,948,526 2,945 2,879
MJDG00000000 SRR1815441 BCW_2991 93 27 3,007,415 3,021 2,958
MJDH00000000 SRR1815442 BCW_2995 91 31 3,005,169 3,017 2,957
MJND00000000 SRR1815443 BCW_2996 107 25 3,003,951 3,020 2,959
MJNE00000000 SRR1815444 BCW_2997 92 17 2,857,564 2,878 2,807
MJOD00000000 SRR1805510 BCW_3811 68 20 2,977,523 2,972 2,912
MJOE00000000 SRR1805407 BCW_3812 48 12 2,841,618 2,828 2,770
MKMD00000000 SRR1815876 BCW_3813 45 19 2,965,876 2,964 2,903
MKME00000000 SRR1815877 BCW_3814 37 23 3,006,902 3,016 2,955
MKMF00000000 SRR1815878 BCW_3815 35 16 2,842,153 2,843 2,784
MKMG00000000 SRR1815879 BCW_3816 37 20 2,928,884 2,886 2,825
MKMH00000000 SRR1815880 BCW_3817 35 19 2,914,544 2,898 2,839
MKMI00000000 SRR1815881 BCW_3818 28 17 2,878,888 2,862 2,804
MKMJ00000000 SRR1815882 BCW_3819 73 21 2,971,782 2,961 2,900
MKMK00000000 SRR1815883 BCW_3820 40 16 2,868,238 2,857 2,798
MKML00000000 SRR1815884 BCW_3821 31 12 2,859,251 2,837 2,787
MKMM00000000 SRR1815885 BCW_3822 39 28 2,917,738 2,912 2,851
MKMN00000000 SRR1815886 BCW_3823 41 22 2,961,674 2,970 2,907
MKMO00000000 SRR1815887 BCW_3824 52 38 3,009,760 3,034 2,972
MKMP00000000 SRR1815888 BCW_3825 39 14 2,897,778 2,883 2,825
MKMQ00000000 SRR1815889 BCW_3826 64 12 2,892,501 2,860 2,799
MKMR00000000 SRR1815890 BCW_3827 41 17 2,902,289 2,884 2,819
MKMS00000000 SRR1815891 BCW_3828 80 20 2,981,768 3,008 2,950
MKMT00000000 SRR1815892 BCW_3829 33 21 2,867,338 2,828 2,773
MKMU00000000 SRR1815893 BCW_3830 30 12 2,873,745 2,826 2,766
MKMV00000000 SRR1815894 BCW_3831 37 13 2,822,641 2,777 2,716
MKMW00000000 SRR1815895 BCW_3832 32 14 2,885,730 2,863 2,806
MKMX00000000 SRR1815896 BCW_3833 35 25 2,941,985 2,933 2,878
MKMY00000000 SRR1815897 BCW_3834 32 17 2,966,648 2,991 2,935
MKMZ00000000 SRR1815898 BCW_3835 49 10 2,857,933 2,803 2,743
MKNA00000000 SRR1815899 BCW_3836 85 11 2,873,808 2,860 2,800
MKNB00000000 SRR1815900 BCW_3837 49 7 2,903,394 2,872 2,818
MKNC00000000 SRR1815901 BCW_3838 54 10 2,846,174 2,821 2,765
MKND00000000 SRR1815902 BCW_3839 67 19 2,893,770 2,869 2,810
MKNE00000000 SRR1815903 BCW_3840 60 24 2,979,754 2,978 2,921
MKNF00000000 SRR1815904 BCW_3841 68 12 2,852,100 2,838 2,775
MKNG00000000 SRR1815905 BCW_3842 56 11 2,835,578 2,818 2,759
MKNH00000000 SRR1815906 BCW_3843 73 14 2,870,259 2,848 2,792
MKNI00000000 SRR1815907 BCW_3844 83 9 2,918,129 2,921 2,861
MKNJ00000000 SRR1815908 BCW_3845 53 9 2,874,832 2,844 2,784
MKNK00000000 SRR1815909 BCW_3846 315 13 2,927,242 2,918 2,866
MKNL00000000 SRR1815910 BCW_3847 37 12 2,905,480 2,900 2,837
MKNM00000000 SRR1815911 BCW_3848 43 6 2,873,840 2,840 2,780
MKNN00000000 SRR1815912 BCW_3849 52 7 2,838,591 2,802 2,742
MKNO00000000 SRR1815913 BCW_3850 50 15 2,860,446 2,857 2,796
MKNP00000000 SRR1815914 BCW_3851 91 11 2,909,975 2,916 2,860
MKNQ00000000 SRR1815915 BCW_3852 87 8 2,907,378 2,882 2,825
MKNR00000000 SRR1815916 BCW_3853 121 13 2,854,561 2,799 2,746
MKNS00000000 SRR1815917 BCW_3854 237 13 2,858,772 2,797 2,750
MKNT00000000 SRR1815918 BCW_3855 71 7 2,884,000 2,859 2,800
MKNU00000000 SRR1815919 BCW_3856 43 16 2,840,147 2,822 2,762
MKNV00000000 SRR1815920 BCW_3857 82 12 2,918,757 2,941 2,881
MKNW00000000 SRR1815921 BCW_3858 78 15 2,864,782 2,844 2,778
MKNX00000000 SRR1815922 BCW_3859 49 12 2,897,160 2,890 2,833
MKNY00000000 SRR1815923 BCW_3860 49 9 2,844,019 2,805 2,740
MKNZ00000000 SRR1815924 BCW_3861 37 15 2,820,199 2,780 2,719
MKOA00000000 SRR1815925 BCW_3862 25 12 2,870,692 2,822 2,766
MKOB00000000 SRR1815926 BCW_3863 18 39 2,943,002 2,954 2,892
MKOC00000000 SRR1815927 BCW_3864 51 16 2,854,724 2,833 2,773
MKOD00000000 SRR1815928 BCW_3865 29 19 2,926,734 2,927 2,868
MKOE00000000 SRR1815929 BCW_3866 27 21 2,943,390 2,974 2,917
MKOF00000000 SRR1815930 BCW_3867 45 12 2,896,382 2,890 2,829
MKOG00000000 SRR1815931 BCW_3868 41 15 2,891,637 2,891 2,833
MKOH00000000 SRR1815932 BCW_3869 31 16 2,956,706 2,980 2,920
MKOI00000000 SRR1815933 BCW_3870 25 29 2,828,150 2,838 2,788
MKOJ00000000 SRR1815934 BCW_3871 21 37 3,081,204 3,115 3,063
MKOK00000000 SRR1815935 BCW_3872 18 22 2,803,150 2,833 2,775
MKOL00000000 SRR1815936 BCW_3873 27 15 2,837,427 2,820 2,766
MKOM00000000 SRR1815937 BCW_3874 20 60 2,958,576 2,962 2,909
MKON00000000 SRR1815938 BCW_3875 57 18 2,888,109 2,891 2,835
MKOO00000000 SRR1815939 BCW_3876 46 22 2,922,426 2,922 2,863
MKOP00000000 SRR1815940 BCW_3877 31 36 3,087,006 3,125 3,066
MKOQ00000000 SRR1815941 BCW_3878 28 24 2,909,010 2,874 2,819
MKOR00000000 SRR1815942 BCW_3879 18 52 2,874,381 2,842 2,786
MKOS00000000 SRR1815943 BCW_3880 24 49 3,004,145 3,051 2,994
MKOT00000000 SRR1815944 BCW_3881 60 17 2,940,478 2,935 2,874
MKOU00000000 SRR1815945 BCW_3882 47 17 2,910,965 2,882 2,811
MKOV00000000 SRR1815946 BCW_3883 55 22 2,905,998 2,883 2,825
MKOW00000000 SRR1815947 BCW_3884 54 23 2,939,753 2,919 2,853
MKOX00000000 SRR1815948 BCW_3885 39 22 2,950,478 2,953 2,885
MKOY00000000 SRR1815949 BCW_3886 30 28 3,071,194 3,048 2,992
MKOZ00000000 SRR1815950 BCW_3887 32 27 2,940,770 2,909 2,850
MKPA00000000 SRR1815951 BCW_3888 45 19 2,947,241 2,948 2,881
MKPB00000000 SRR1815952 BCW_3889 31 18 2,926,393 2,921 2,860
MKPC00000000 SRR1815953 BCW_3890 59 10 2,856,568 2,827 2,765
MKPD00000000 SRR1815954 BCW_3891 39 20 2,967,252 2,986 2,922
MKPE00000000 SRR1815955 BCW_3892 33 18 2,920,115 2,901 2,844
MKPF00000000 SRR1815956 BCW_3893 30 14 2,849,771 2,835 2,779
MKPG00000000 SRR1815957 BCW_3894 29 12 2,882,875 2,874 2,818
MKPH00000000 SRR1815958 BCW_3895 32 15 2,894,936 2,889 2,833
MKPI00000000 SRR1815959 BCW_3896 60 12 2,970,597 2,955 2,894
MKPJ00000000 SRR1815960 BCW_3897 35 18 2,982,868 3,005 2,943
MKPK00000000 SRR1815961 BCW_3898 51 14 2,894,195 2,861 2,801
MKPL00000000 SRR1815962 BCW_3899 48 13 2,860,355 2,852 2,788
MKPM00000000 SRR1815963 BCW_3900 25 23 2,982,615 2,966 2,911
MKPN00000000 SRR1815964 BCW_3901 48 12 2,841,873 2,831 2,774
MKPO00000000 SRR1815965 BCW_3902 43 14 2,931,100 2,950 2,892
MKPP00000000 SRR1815966 BCW_3903 25 15 2,868,643 2,848 2,797
MKPQ00000000 SRR1815967 BCW_3904 36 37 3,035,356 3,050 2,991
MKPR00000000 SRR1815968 BCW_3905 34 14 2,830,709 2,804 2,749
MKPS00000000 SRR1815969 BCW_3906 37 17 2,878,382 2,865 2,807
MJNF00000000 SRR1815970 BCW_3907 63 17 2,928,223 2,918 2,861
MJNG00000000 SRR1815971 BCW_3908 49 24 3,063,990 3,057 2,999
MJNH00000000 SRR1815972 BCW_3909 40 11 2,839,271 2,817 2,763
MJNI00000000 SRR1815973 BCW_3910 47 17 2,758,147 2,771 2,717
MJNJ00000000 SRR1815974 BCW_3911 63 18 2,926,597 2,906 2,845
MJNK00000000 SRR1815975 BCW_3912 49 15 2,852,829 2,837 2,777
MJNL00000000 SRR1815976 BCW_3913 52 17 2,875,683 2,884 2,826
MJNM00000000 SRR1815977 BCW_3914 48 23 2,921,018 2,928 2,868
MJNN00000000 SRR1815978 BCW_3915 40 20 2,875,515 2,848 2,793
MJNO00000000 SRR1815979 BCW_3916 41 18 2,999,138 2,995 2,941
MJNP00000000 SRR1815980 BCW_3917 72 15 2,837,435 2,824 2,765
MJNQ00000000 SRR1815981 BCW_3918 45 17 2,889,824 2,889 2,832
MJNR00000000 SRR1815982 BCW_3919 36 18 2,914,631 2,912 2,856
MJNS00000000 SRR1815983 BCW_3920 44 21 2,902,374 2,865 2,804
MJNT00000000 SRR1815984 BCW_3921 31 21 2,906,315 2,890 2,835
MJNU00000000 SRR1815985 BCW_3922 56 11 2,878,388 2,880 2,821
MJNV00000000 SRR1815986 BCW_3923 46 20 2,927,260 2,959 2,900
MJNW00000000 SRR1815987 BCW_3924 81 19 2,897,709 2,863 2,799
MJNX00000000 SRR1815988 BCW_3925 86 24 3,014,318 3,024 2,964
MJNY00000000 SRR1815989 BCW_3926 54 24 3,056,181 3,071 3,011
MJNZ00000000 SRR1815990 BCW_3927 60 16 2,890,051 2,869 2,810
MJOA00000000 SRR1815991 BCW_3928 58 23 3,122,409 3,159 3,098
MJOB00000000 SRR1815992 BCW_3929 44 21 2,950,233 2,957 2,898
MJOC00000000 SRR1815993 BCW_3930 44 20 2,941,469 2,924 2,861
MJOF00000000 SRR1815994 BCW_3931 90 15 2,889,246 2,870 2,813
MJOG00000000 SRR1815995 BCW_3932 74 19 2,975,068 2,972 2,911
MJOH00000000 SRR1815996 BCW_3933 68 10 2,822,712 2,787 2,731
MJOI00000000 SRR1815997 BCW_3934 66 10 2,829,488 2,795 2,736
MJOJ00000000 SRR1815998 BCW_3935 28 190 3,149,703 3,194 3,138
MJOK00000000 SRR1805379 BCW_3936 39 17 2,737,049 2,725 2,669
MJOL00000000 SRR1816001 BCW_3938 57 18 2,879,444 2,851 2,790
MJOM00000000 SRR1816002 BCW_3939 64 14 2,878,718 2,887 2,823
MJON00000000 SRR1816003 BCW_3940 41 14 2,859,559 2,835 2,776
MJOO00000000 SRR1816004 BCW_3941 86 26 3,074,403 3,087 3,026
MJOP00000000 SRR1816005 BCW_3942 64 18 2,973,453 2,970 2,911
MJOQ00000000 SRR1816006 BCW_3943 43 19 2,962,794 2,980 2,921
MJOR00000000 SRR1816007 BCW_3944 74 32 3,093,581 3,165 3,104
MJOS00000000 SRR1816008 BCW_3945 57 23 2,959,411 3,001 2,943
MJOT00000000 SRR1816053 BCW_4252 41 18 2,904,951 2,915 2,858
MJOU00000000 SRR1816054 BCW_4253 52 29 3,053,325 3,071 3,012
MJOV00000000 SRR1816055 BCW_4254 77 28 3,104,645 3,137 3,079
MJOW00000000 SRR1816056 BCW_4255 64 23 2,935,300 2,917 2,856
MJOX00000000 SRR1816057 BCW_4256 51 34 3,142,820 3,178 3,119
MJOY00000000 SRR1816058 BCW_4257 66 14 2,935,555 2,923 2,862
MJOZ00000000 SRR1816059 BCW_4258 51 25 3,096,969 3,103 3,044
MJPA00000000 SRR1816060 BCW_4259 68 26 2,980,170 2,966 2,905
MJPB00000000 SRR1816061 BCW_4260 48 34 3,013,184 3,016 2,956
MJPC00000000 SRR1816062 BCW_4261 119 29 3,148,803 3,157 3,101
MJPD00000000 SRR1816063 BCW_4262 39 49 3,159,840 3,175 3,119
MJPE00000000 SRR1816064 BCW_4263 47 35 3,165,508 3,198 3,136
MJPF00000000 SRR1816065 BCW_4264 66 14 2,967,285 2,951 2,890
MJPG00000000 SRR1816066 BCW_4265 41 13 3,002,824 2,998 2,935
MJPH00000000 SRR1816067 BCW_4266 47 19 3,028,588 3,054 2,998
MJPI00000000 SRR1816068 BCW_4267 58 22 3,004,577 3,011 2,952
MJPJ00000000 SRR1816069 BCW_4268 35 34 3,044,813 3,053 3,003
MJPK00000000 SRR1816070 BCW_4269 49 14 2,930,225 2,961 2,904
MJPL00000000 SRR1816071 BCW_4270 37 24 3,057,295 3,107 3,046
MJPM00000000 SRR1816072 BCW_4271 43 17 3,003,458 3,004 2,943
MJPN00000000 SRR1816073 BCW_4272 36 36 3,059,631 3,083 3,024
MJPO00000000 SRR1816074 BCW_4273 47 32 3,056,980 3,069 3,007
MJPP00000000 SRR1816075 BCW_4274 35 29 3,092,169 3,142 3,083
MJPQ00000000 SRR1816076 BCW_4275 53 17 2,970,143 2,969 2,910
MJPR00000000 SRR1816077 BCW_4276 30 20 3,023,906 3,032 2,975
MJPS00000000 SRR1816078 BCW_4277 55 10 2,932,904 2,947 2,887
MJPT00000000 SRR1816079 BCW_4278 66 22 2,969,319 3,003 2,949
MJPU00000000 SRR1816080 BCW_4279 61 16 2,959,605 2,941 2,884
MJPV00000000 SRR1816081 BCW_4280 34 18 2,956,561 2,976 2,920
MJPW00000000 SRR1816082 BCW_4281 41 24 2,999,322 2,990 2,934
MJPX00000000 SRR1816083 BCW_4282 128 19 3,053,557 3,069 3,017
MJPY00000000 SRR1816084 BCW_4283 182 34 2,958,989 2,952 2,899
MJPZ00000000 SRR1816085 BCW_4284 150 36 3,126,895 3,167 3,107
MJQA00000000 SRR1816086 BCW_4285 43 19 2,933,555 2,909 2,848
MJQB00000000 SRR1816088 BCW_4287 94 18 3,004,278 3,013 2,956
MJQC00000000 SRR1816089 BCW_4288 47 29 3,087,349 3,115 3,058
MJQD00000000 SRR1816090 BCW_4289 145 15 2,947,025 2,955 2,900
MJQE00000000 SRR1816091 BCW_4290 70 12 3,001,188 2,998 2,939
MJQF00000000 SRR1816092 BCW_4291 161 20 3,084,559 3,109 3,048
MJQG00000000 SRR1816093 BCW_4292 163 28 3,015,259 3,039 2,984
MJQH00000000 SRR1816094 BCW_4293 53 26 3,011,157 3,015 2,955
MJQI00000000 SRR1816095 BCW_4294 44 39 3,008,725 3,024 2,965
MJQJ00000000 SRR1816096 BCW_4295 30 48 3,080,304 3,114 3,057
MJQK00000000 SRR1816097 BCW_4296 32 14 2,829,770 2,814 2,758
MJQL00000000 SRR1816098 BCW_4297 52 25 3,078,586 3,116 3,056
MJQM00000000 SRR1816099 BCW_4298 162 16 2,985,857 2,976 2,926
MJQN00000000 SRR1816100 BCW_4299 55 20 2,965,573 2,991 2,931
MJQO00000000 SRR1816101 BCW_4300 58 14 2,890,745 2,872 2,814
MJQP00000000 SRR1816102 BCW_4301 72 39 3,129,421 3,198 3,138
MJQQ00000000 SRR1816103 BCW_4302 59 19 2,967,831 2,955 2,896
MJQR00000000 SRR1816105 BCW_4304 52 23 2,981,718 2,976 2,917
MJQS00000000 SRR1816106 BCW_4305 17 31 2,904,001 2,902 2,854
MJQT00000000 SRR1816107 BCW_4306 92 34 3,012,898 3,022 2,963
MJQU00000000 SRR1816108 BCW_4307 70 17 2,979,023 2,982 2,924
MJQV00000000 SRR1816109 BCW_4308 48 30 3,149,424 3,149 3,091
MJQW00000000 SRR1805452 BCW_4309 541 29 2,964,367 2,973 2,925
MJQX00000000 SRR1805386 BCW_4311 82 16 2,990,843 3,032 2,974
MJQY00000000 SRR1805458 BCW_4312 66 17 2,989,606 2,988 2,933
MJQZ00000000 SRR1805418 BCW_4313 130 15 2,844,766 2,828 2,775
MJSH00000000 SRR1805444 BCW_4314 386 21 2,987,238 2,963 2,917
MJRA00000000 SRR2982244 BCW_4315 82 6 2,870,740 2,846 2,785
MJRB00000000 SRR1805544 BCW_4316 85 19 3,061,290 3,084 3,023
MJRC00000000 SRR1816370 BCW_4758 79 16 2,988,627 2,995 2,935
MJRD00000000 SRR1816372 BCW_4760 94 11 2,879,424 2,878 2,824
MJRE00000000 SRR1816373 BCW_4761 89 12 2,951,127 2,969 2,914
MJRF00000000 SRR1816374 BCW_4762 56 18 3,078,289 3,109 3,054
MJRG00000000 SRR1816375 BCW_4763 39 23 3,005,047 3,038 2,977
MJRH00000000 SRR1816376 BCW_4764 84 12 2,953,054 2,969 2,913
MJRI00000000 SRR1816377 BCW_4765 82 12 2,882,576 2,881 2,823
MJRJ00000000 SRR1816378 BCW_4766 82 25 2,906,481 2,882 2,810
MJRK00000000 SRR1816379 BCW_4767 51 16 2,972,582 2,956 2,892
MJRL00000000 SRR1816380 BCW_4768 48 19 2,986,043 3,008 2,952
MJRM00000000 SRR1816381 BCW_4769 54 21 2,937,535 2,904 2,842
MJRN00000000 SRR1816382 BCW_4770 47 21 2,932,710 2,904 2,841
MJRO00000000 SRR1816383 BCW_4771 37 25 2,931,128 2,904 2,843
MJRP00000000 SRR1816384 BCW_4772 52 18 2,978,435 2,976 2,915
MJRQ00000000 SRR1816385 BCW_4773 44 18 2,890,220 2,870 2,810
MJRR00000000 SRR1816386 BCW_4774 35 32 2,939,871 2,963 2,905
MJRS00000000 SRR1816387 BCW_4775 68 17 2,936,512 2,902 2,837
MJRT00000000 SRR1816388 BCW_4776 47 9 2,879,862 2,885 2,825
MJRU00000000 SRR1816389 BCW_4777 48 19 3,005,117 3,025 2,962
MJRV00000000 SRR1816390 BCW_4778 46 14 2,951,95,2 2,973 2,914
MJRW00000000 SRR1816392 BCW_4780 55 15 2,934,901 2,926 2,866
MJRX00000000 SRR1816393 BCW_4781 57 19 3,026,964 3,036 2,974
MJRY00000000 SRR1816394 BCW_4782 42 16 2,941,141 2,949 2,893
MJRZ00000000 SRR1816395 BCW_4783 70 19 2,938,896 2,953 2,895
MJSA00000000 SRR1816396 BCW_4784 64 14 2,954,405 2,961 2,898
MJSB00000000 SRR1816397 BCW_4785 65 20 2,985,984 2,982 2,917
MJSC00000000 SRR1816398 BCW_4786 50 16 2,960,119 2,946 2,884
MJSD00000000 SRR1816399 BCW_4787 42 22 2,980,365 2,984 2,920
MJSE00000000 SRR1816400 BCW_4788 55 19 2,929,400 2,894 2,836
MJSF00000000 SRR1816401 BCW_4789 47 8 2,884,261 2,888 2,831
MJSG00000000 SRR1816402 BCW_4790 44 26 3,008,528 3,038 2,975
Avg 135 20 2,948,523 2,950 2,891
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a

CDSs, coding sequences.

ACKNOWLEDGMENTS

We thank Lucy Cai, Alvin Leonardo, Patrick Ancheta, Regina Agulto, Vivian Lee, Christina Kong, Kendra Liu, and Derek Yip for their technical assistance.

This project was funded by the 100K Pathogen Genome Project, with initial funding from the FDA, Agilent Technologies, and Mars, Inc. to produce these sequences.

Footnotes

Citation Chen P, Kong N, Huang B, Thao K, Ng W, Storey DB, Arabyan N, Foutouhi A, Foutouhi S, Weimer BC. 2017. 100K Pathogen Genome Project: 306 Listeria draft genome sequences for food safety and public health. Genome Announc 5:e00967-16. https://doi.org/10.1128/genomeA.00967-16.

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