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. Author manuscript; available in PMC: 2017 Mar 10.
Published in final edited form as: Oncogene. 2016 Aug 29;36(10):1394–1403. doi: 10.1038/onc.2016.305

Figure 7.

Figure 7

Inhibition of PI3K/AKT signaling downregulates Vimentin expression. Analysis of the effects of a PI3K inhibitor on the expression of Vimentin was analyzed. MVT-1 cells were serum starved overnight and pretreated for 1 hour with either vehicle or 500nM of NVP-BKM120 (BKM120). Subsequently, cells were stimulated with 10nM of insulin for 48 hours (n=3 per condition, repeated 3 times). Protein was extracted from the cells and analyzed by Western blotting for (A) Vimentin expression and for (B) phosphorylated AKT and total AKT. β-Actin was used as a loading control. (B, D) Densitometry of western blots A and C, respectively (* p<0.05). Graphs represent mean per group and error bars are SEM.