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. 1990 Jan;87(2):709–713. doi: 10.1073/pnas.87.2.709

Transcription and cap trimethylation of a nematode spliced leader RNA in a cell-free system.

P A Maroney 1, G J Hannon 1, T W Nilsen 1
PMCID: PMC53335  PMID: 2300557

Abstract

Maturation of a fraction of mRNAs in nematodes involves the acquisition of a common 5' terminal spliced leader sequence derived from a nonpolyadenylylated spliced leader RNA by trans splicing. We have developed a cell-free system prepared from Ascaris lumbricoides embryos that accurately and efficiently synthesized the spliced leader RNA of A. lumbricoides. Transcription of the spliced leader RNA was catalyzed by RNA polymerase II, and the majority of the spliced leader RNAs synthesized in vitro possessed a trimethylguanosine cap structure identical to that found on in vivo-synthesized spliced leader RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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