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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Nature. 2017 Feb 8;543(7643):122–125. doi: 10.1038/nature21356

Fig. 1. Integrated next-generation DNA sequencing and cytogenetic analysis of ECDNA.

Fig. 1

a, Schematic diagram of experimental flow. b, Representative metaphases stained with DAPI and a genomic DNA FISH probe (ECDNA, arrows). c, DNase treatment abolishes DAPI staining of chromosomal and ECDNA (arrows). d, Pan-centromeric FISH reveals absence of a centromere in ECDNAs (arrows). e, Schematic illustration of ECdetect. e.1) DAPI-stained metaphase as input. e.2) Semi-automated identification of ECDNA search region via segmentation. e.3) Conservative filtering, removing non-ECDNA components. e.4) ECDNA detection and visualization. (F). Pearson correlation between software-detected and manual calls of ECDNA (R: 0.98, p < 2.2 × 10−16.