Abstract
We herein report a case of acute cholangitis and bacteremia caused by a commensal Neisseria species, Neisseria subflava, in an 82-year-old man with cholangiocarcinoma. Emergency endoscopic nasobiliary drainage and cefoperazone/sulbactam therapy were effective. Gram negative coccobacilli were isolated from both blood and bile cultures on 5% sheep blood agar. The isolate was identified as N. subflava biovar perflava by mass spectrometry, a sequence analysis of the 16S rRNA, and biochemical testing. Although biliary infections due to commensal Neisseria are extremely rare, this case demonstrates the possibility of its occurrence in patients undergoing bile duct treatment.
Keywords: Neisseria subflava biovar perflava, acute cholangitis, bacteremia, endoscopic retrograde cholangiopancreatography, cholangiocarcinoma
Introduction
Neisseria subflava is a family of the so-called commensal Neisseria that is a part of the normal oral flora. Commensal Neisseria is a rare cause of invasive diseases such as meningitis, endocarditis, bacteremia, ocular infections, pericarditis, empyema, peritonitis, septic arthritis, bursitis, and osteomyelitis (1). Reports of intraabdominal infection due to Neisseria species are exceedingly rare. Among these, a form of perihepatitis caused by N. gonorrhoeae, referred to as the Fitz-Hugh-Curtis syndrome, is well-characterized. A few cases of peritoneal dialysis-related peritonitis caused by Neisseria species have been reported (2,3). However, there are no reported cases of infection of the biliary system by Neisseria species. We herein report a case of acute cholangitis and bacteremia due to N. subflava.
Case Report
An 82-year-old man with cholangiocarcinoma was admitted to the emergency room of the Keio University Hospital for acute epigastric pain and vomiting. The patient's medical history was unremarkable, except for hypertension and lumbar stenosis, until the dilation of a lower bile duct was detected on a positron emission tomography scan at an annual health check. One month prior to admission, he had been diagnosed with distal extrahepatic bile duct cancer after diagnostic endoscopic retrograde cholangiopancreatography (ERCP) was performed a second time.
At admission, the patient was febrile (38.4℃) but his other vital signs were stable. There was epigastric tenderness and a yellowish discoloration of the skin. Laboratory tests showed elevated liver enzyme levels (AST; 589 IU/L, ALT; 307 IU/L, LDH; 620 IU/L, ALP; 410 IU/L) and hyperbilirubinemia (Total bilirubin; 3.4 mg/dL). The patient's white blood cell counts were normal. Computed tomography (CT) revealed a dilated common bile duct. He was diagnosed with acute cholangitis, and cefoperazone/sulbactam was administered after obtaining two sets of blood cultures (BD, Franklin Lakes, NJ, USA).
Emergency endoscopic nasobiliary drainage was performed, and a bile specimen was obtained. After 7 days of antibiotic treatment, he gradually improved and was discharged. Radical pancreatoduodenectomy was performed 8 days later.
Two sets of blood cultures showed bacterial growth after 9 hours of incubation. Gram staining showed Gram negative coccobacilli. Gram negative coccobacilli were also isolated from a bile culture on 5% sheep blood agar (Nissui, Tokyo, Japan).
Samples from these positive blood cultures were subcultured on 5% sheep blood agar (Nissui) and MacConkey II agar (BD) under aerobic conditions at 35℃. The colonies were slightly yellow, smooth, opaque, and often adherent. The Gram staining of the colonies showed Gram negative diplococci.
Bacterial identification using a BBL Crystal™ Neisseria/Haemophilus identification system (BD) indicated N. subflava. Mass spectrometry using AXIMA™ Performance (Shimadzu, Kyoto, Japan), and VITEK™ MS plus systems (Sysmex bioMerieux, Lyon, France) showed a 99.9% morphology match with N. subflava. To confirm the identity of the isolates, genomic DNA was extracted from the colonies by heat extraction (100℃, 10 min); PCR amplification and sequencing were performed to characterize the 16S rRNA gene. An analysis using the Blast software program revealed the strong homology (>99%) of the obtained 16S rRNA gene sequences (1,220-1,331 bp) with N. subflava biovar perflava; these were found to be 99.85% identical to those of N. subflava biovar perflava U15 strain in an analysis using the EzTaxon software program (http://www.ezbiocloud.net/eztaxon/). However, the same gene sequences were found to be 99.62% identical to N. flavescens ATCC13120 strain, N. mucosa M5 strain in an analysis using the EzTaxon software program.
To distinguish between these three species, biochemical testing was performed using ID Test HN-20 Rapid tools (Nissui) according to manufacturer's instructions. The results (Table) were positive for acid production from glucose, maltose, sucrose, and fructose; however, they were negative for acid production from lactose and nitrate reduction. These results were compatible with the biochemical activity profile of N. subflava biovar perflava and different from the profiles of N. flavescens, and N. mucosa (4). Thus, the Gram negative isolates recovered from the blood and bile cultures were determined to be N. subflava biovar perflava. Based on the results, the patient was diagnosed with acute cholangitis and bacteremia caused by N. subflava biovar perflava.
Table.
Biochemical Chalacteristics of Current Strain and Neisseria Species4.
Species | Acid production from: | Nitrate reduction |
|||
---|---|---|---|---|---|
GLU | MAL | SUC | FRU | ||
N. flavescens | - | - | - | - | - |
N. mucosa | + | + | + | + | + |
N.subflava | |||||
bv. flava | + | + | - | + | - |
bv. subflava | + | + | - | - | - |
bv. perflava | + | + | + | + | - |
Current strain | + | + | + | + | - |
GLU: glucose, MAL: maltose, SUC: sucrose, FRU: fructose.
Discussion
N. subflava was isolated from both blood and bile cultures from a patient with acute cholangitis with cholangiocarcinoma. N. subflava has low pathogenicity. A literature search of the PubMed database suggested that documented cases of N. subflava blood stream infection have largely been limited to cases of meningitis (5), endocarditis (6), and osteomyelitis (7). Most of the cases occurred after medical intervention or intravenous drug injections. Although further testing is needed to confirm the identification, it is not difficult to identify N. subflava using a widely available kit. Thus, N. subflava blood stream infections are not likely to be overlooked at ordinary clinical laboratories. The small number of reports on N. subflava blood stream infections is simply due to the rarity of N. subflava blood stream infections.
In the present case, the minimum inhibitory concentration (MIC) of N. subflava isolated from blood culture was measured using the broth microdilution method. The MICs for penicillin G and cefotaxime were 0.5 μg/mL and <0.5 μg/mL, respectively. Although antimicrobial breakpoints against N. subflava have not been determined, the isolate was classified as having intermediate resistance to penicillin G and susceptibility to cefotaxime according to the breakpoint criteria for N. gonorrhoeae in CLSI M100-S26 (8). This result is consistent with a previous domestic report that showed reduced susceptibility against penicillin G (9). In this case, cefoperazone/sulbactam, a third generation cephalosporin, was administered. Although the MIC for cefoperazone/sulbactam was not measured, the antimicrobial therapy was effective in the present case because of the susceptibility to cefotaxime.
N. subflava is a component of the normal oral flora; it is not commonly found in the bile tract or duodenum. The common pathogens causing biliary tract infection are enterobacteriaceae, enterococci, and anaerobes (10), which normally inhabit the gastrointestinal tract. In this case, it was not exactly clear why N. subflava caused cholangitis. We hypothesize that the diagnostic ERCP performed as a part of a diagnostic work-up for bile duct neoplasm might have played a role. The normal oral flora, including N. subflava, may have been displaced to the duodenum and bile duct. The biliary obstruction due to the progression of cholangiocarcinoma may have finally caused cholangitis due to presence of the N. subflava colonies in the duodenum. Furthermore, endoscopic sphincterotomy was performed at the second diagnostic ERCP trial, which might have rendered the biliary sphincter permanently insufficient. The loss of this physiologic barrier between the duodenum and biliary tract results in duodenocholedochol reflux and bacterial colonization of the biliary tract (11). Colonization of the biliary tract and cholangitis were more likely to have occurred in this patient. This study is associated with two major limitations. First, there was no evidence that N. subflava, which was isolated from the blood, was originally derived from components of the normal oral flora because no oral cultures were obtained. However, in a previous study, 37 out of 40 healthy adults were found to carry N. subflava biovar perflava in their nasopharynx (12). Secondly, there are no documented cases of post-ERCP duodenal and biliary colonization by Neisseria or post-ERCP cholangitis due to oral normal flora. However, a case of post-ERCP retroperitoneal abscess caused by Haemophilus parainfluenza, a component of the normal oral flora is on record (13).
Further research on cholangitis due to Neisseria is necessary to confirm this hypothesis. To the best of our knowledge this is the first report of cholangitis due to Neisseria species, which demonstrates that commensal Neisseria can cause cholangitis in a patient after bile duct treatment.
Funding sourse This study was funded by Keio University School of Medicine.
The authors state that they have no Conflict of Interest (COI).
References
- 1. Murphy TF. Moraxella catarrhalis, Kingella, and Other Gram-Negative Cocci. In: Principles and Practice of Infectious Diseases. 7th ed. Mandell GL, Bennett JE, Dolin R, et al. , Eds. Philadelphia Churchill Livingstone Elsevier, Philadelphia, 2010: 2771-2776. [Google Scholar]
- 2. Shelty AK, Nagarj SK, Lorentz WB, Bitzan M. Peritonitis due to Neisseria mucosa in an adolescent receiving peritoneal dialysis. Infection 33: 390-392, 2005. [DOI] [PubMed] [Google Scholar]
- 3. Kocyigit I, Unal A, Sipahioglu M, Tokgoz B, Oymak O, Utas C. Peritoneal dialysis-related peritonitis due to Neisseria weaveri: the first case report. Perit Dial Int 30: 116-117, 2010. [DOI] [PubMed] [Google Scholar]
- 4. Elias J, Frosch M, Vogel U. Neisseria. In: Manual of Clinical Microbiology. 11th ed. Jorgensen JH, Pfalle MA, Carrel KC, et al. , Eds. ASM Press, Washington DC, 2015: 635-651. [Google Scholar]
- 5. Entesari-Tatafi D, Bagherirad M, Quan D, Athan E. Iatrogenic meningitis caused by Neisseria sicca/subflava after intrathecal contrast injection, Australia. Emerg Infect Dis 20: 1023-1025, 2014. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6. Amsel BJ, Moulijn AC. Nonfebrile mitral valve endocarditis due to Neisseria subflava. Chest 109: 280-282, 1996. [DOI] [PubMed] [Google Scholar]
- 7. Assimacopoulos AP. Epidural abscess, discitis and vertebral osteomyelitis caused by Neiserria subflava. S D J Med 65: 265-269, 2007. [PubMed] [Google Scholar]
- 8. Clinical and Laboratory Standards Institute. . CLSI supplement M100S. In: Performance Standards for Antimicrobial Susceptibility Testing. 26th ed. Clinical and Laboratory Standards Institute, Wayne, 2016. [Google Scholar]
- 9. Furuya R, Onoye Y, Kanayama A, et al. Antimicrobial resistance in clinical isolates of Neisseria subflava from the oral cavities of a Japanese population. J Infect Chemother 13: 302-304, 2007. [DOI] [PubMed] [Google Scholar]
- 10. Gomi H, Solomkin JS, Takeda T, et al. TG13 antimicrobial therapy for acute cholangitis and cholecystitis. J Hepatobiliary Pancreat Sci 20: 60-70, 2013. [DOI] [PubMed] [Google Scholar]
- 11. Zhang ZH, Wu YG, Qin CK, et al. Severe acute cholangitis after endoscopic sphincterotomy induced by barium examination: a case report. World J Gastroenterol 18: 5658-5660, 2012. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12. Sáez Nieto JA, Marcos C, Vindel A. Multicolonization of human nasopharynx due to Neisseria spp. Int Microbiol 1: 59-63, 1998. [PubMed] [Google Scholar]
- 13. Patel SB, Hashimi ZA, Marx RJ. A retroperitoneal abscess caused by Haemophilus parainfluenza after endoscopic retrograde cholangiopancreatography and open cholecystectomy with a common bile duct exploration: a case report. J Med Case Rep 4: 170-172, 2010. [DOI] [PMC free article] [PubMed] [Google Scholar]