Significance
Thyroid cancer is the fastest-growing cancer type in the United States, yet its molecular mechanisms are not fully understood. Here we report the identification and characterization of a gene fusion involving the thyroid adenoma-associated (THADA) and LOC389473 genes in papillary thyroid cancers, which leads to strong overexpression of insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) and signaling via the IGF2/IGF1 receptor (IGF1R) pathway. As a result, we report a previously unknown structural mechanism responsible for IGF2BP3 overexpression in cancer. We also show that IGF2BP3 overexpression via different mechanisms can be found in 5 to 15% of several cancer types, and demonstrate that growth of these tumors can be blocked by IGF1R inhibitors. This raises the possibility that IGF2BP3 overexpression may be used to predict therapeutic response to IGF1R inhibition in cancer cells.
Keywords: thyroid cancer, genetics, chromosomal rearrangements, IGF2BP3, IGF1R
Abstract
Thyroid cancer development is driven by known point mutations or gene fusions found in ∼90% of cases, whereas driver mutations in the remaining tumors are unknown. The insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) plays an important role in cancer, yet the mechanisms of its activation in cancer cells remain poorly understood. Using whole-transcriptome and whole-genome analyses, we identified a recurrent fusion between the thyroid adenoma-associated (THADA) gene on chromosome 2 and the LOC389473 gene on chromosome 7 located 12 kb upstream of the IGF2BP3 gene. We show that THADA fusion to LOC389473 and other regions in the vicinity does not result in the formation of a chimeric protein but instead leads to strong overexpression of the full-length IGF2BP3 mRNA and protein, increased IGF2 translation and IGF1 receptor (IGF1R) signaling via PI3K and MAPK cascades, and promotion of cell proliferation, invasion, and transformation. THADA fusions and IGF2BP3 overexpression are found in ∼5% of thyroid cancers that lack any other driver mutations. We also find that strong IGF2BP3 overexpression via gene fusion, amplification, or other mechanisms occurs in 5 to 15% of several other cancer types. Finally, we provide in vitro and in vivo evidence that growth of IGF2BP3-driven cells and tumors may be blocked by IGF1R inhibition, raising the possibility that IGF2BP3 overexpression in cancer cells may predict an anti-IGF1R benefit.
Genome-wide sequencing analyses offer a valuable tool for better understanding of genetic mechanisms of tumorigenesis and identifying new therapeutic targets for cancer. Such approaches have led to identification of driver mutations in the majority of thyroid cancer cases (1, 2). They confirmed that point mutations and gene fusions leading to the activation of the MAPK and PI3K/AKT signaling pathways are crucial for tumorigenesis and progression of these tumors. Among papillary thyroid carcinomas (PTCs), the most common type of thyroid cancer, ∼75% of tumors have driver point mutations, whereas ∼15% show nonoverlapping chromosomal rearrangements typically leading to the activation of receptor tyrosine kinases (1). Driver point mutations or gene fusions in the remaining PTCs remain unknown.
Typically, oncogenic chromosomal rearrangements result in an in-frame fusion of a 5′ portion of an actively transcribed gene to a functionally important 3′ portion of an oncogene, frequently coding for the kinase domain, leading to gene expression and constitutive kinase activation (3). Other, less common mechanisms include translocation of an oncogene to a region of actively transcribed chromatin, leading to gene activation without formation of the chimeric gene products (4–6). Such events are more difficult to identify as biologically relevant during the analysis of next-generation sequencing data, because no typical in-frame fusion of two coding gene sequences can be found. This may be responsible for a failure to identify driver gene fusions in a significant proportion of common cancers with currently unknown driver mutations (7).
In this study, we used whole-transcriptome (RNA sequencing; RNA-seq) and whole-genome sequencing (WGS) of PTCs to identify recurrent fusions between the thyroid adenoma-associated (THADA) gene on chromosome 2 and a genomic region on chromosome 7 and demonstrate that it does not lead to the formation of the chimeric protein but instead results in strong overexpression of the downstream insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) gene, which is a transforming event in thyroid tumors and a potential therapeutic target for IGF1 receptor (IGF1R) inhibitors in different cancer types.
Results
Identification of THADA–LOC389473 and THADA–IGF2BP3 Fusions.
To identify novel driver events in thyroid cancer, a prescreened group of 25 PTCs lacking all known mutations was studied by RNA-seq (21 tumors) or WGS (4 tumors) using paired-end sequencing on an Illumina HiSeq system. Using RNA-seq, six tumors were found to have gene fusions involving the THADA gene located on 2p21. In four tumors (T1, T2, T3, and T5), RNA-seq results revealed fusions between different exons (28, 29, 30, or 36) of THADA and the noncoding region on 7p15.3 [chromosome (chr.) position 23,521,997] (Fig. 1A and SI Appendix, Table S1). This region is located 12 kb upstream of the IGF2BP3 gene and appears to contain a putative Homo sapiens gene, LOC389473. RNA-seq analysis of the other two tumors revealed fusions between exon 30 of THADA and exon 3 of IGF2BP3 (T4) or between exon 31 of THADA and exon 2 of IGF2BP3 (T6). All fusions were confirmed by RT-PCR and Sanger sequencing (Fig. 1B and SI Appendix, Fig. S1). In addition, upon manual inspection of RNA-seq reads for T4 with the THADA exon 30-to-IGF2BP3 exon 3 fusion, single reads spanning THADA exon 30 and LOC389473 were also identified and confirmed by RT-PCR and Sanger sequencing (SI Appendix, Fig. S1). Using WGS, one additional tumor (T7) was found to have a fusion between intron 31 of THADA and a region located 1.5 kb upstream of the IGF2BP3 gene (chr. position 23,511,508) (Fig. 1A). The fusion was confirmed by PCR and Sanger sequencing of tumor DNA (Fig. 1D), and a reciprocal fusion was detected (Fig. 1E), indicating that it is a result of a balanced translocation between chromosomes 2 and 7. However, no chimeric mRNA involving THADA exon 31 fused with any of the first four IGF2BP3 exons was detected by RT-PCR (SI Appendix, Fig. S2). The t(2;7) rearrangement was further confirmed by FISH in each of the five tumors studied (Fig. 1C and SI Appendix, Fig. S3).
THADA–LOC389473 Results in Overexpression of Downstream IGF2BP3.
The THADA–LOC389473 fusion places the partner genes in a head-to-tail orientation and they transcribe in opposite directions, so the fusion mRNA contains a stop codon downstream of the fusion point in all four tumors (SI Appendix, Fig. S4), suggesting that THADA–LOC389473 is unlikely to yield a functional protein. This, together with finding the THADA–IGF2BP3 fusions in other tumors, allowed us to hypothesize that THADA–LOC389473 may affect carcinogenesis instead via altering the IGF2BP3 gene. To test this, we first detected the expression levels of THADA and IGF2BP3 in normal thyroid tissues and tumors by quantitative (q)RT-PCR. The analysis showed that THADA was expressed at a high level in normal thyroid follicular cells compared with other cell types studied, and its expression was not altered in thyroid cancers positive or negative for THADA fusions (Fig. 2A and SI Appendix, Fig. S5). In contrast, IGF2BP3 was found to be expressed at a very low level in normal thyroid cells and tumors negative for the fusion, but its expression was increased 90- to 102-fold in all tumors carrying the fusion (Fig. 2B). Similar findings were observed in the RNA-seq data, which showed the increased expression of the full-length IGF2BP3 mRNA in tumors positive for THADA–LOC389473 and THADA–IGF2BP3 fusions (Fig. 2C and SI Appendix, Fig. S5). Similarly, by Western blot analysis, all six tumors studied showed high levels of IGF2BP3 protein of ∼69-kDa molecular weight, corresponding to a full-length protein, and no other protein products that would correspond to a chimeric gene product (Fig. 2D). The analysis included tumors with THADA–LOC389473 and THADA–IGF2BP3 fusions and a THADA intron fused to a sequence 1.5 kb upstream of IGF2BP3. Further, immunohistochemical analysis with IGF2BP3 antibody showed strong cytoplasmic immunoreactivity in all studied fusion-positive tumors but not in adjacent normal thyroid tissue (Fig. 2E). These data indicate that fusion between the THADA gene and LOC389473, a predicted gene located 12 kb upstream of the IGF2BP3 gene, is unlikely to produce a functional chimeric gene but instead results in strong overexpression of the full-length IGF2BP3 transcript and protein, likely due to juxtaposition of the IGF2BP3 chromosomal locus on 7p15.3 to the actively transcribed THADA locus on 2p21.
Overexpression of IGF2BP3 Leads to an Increase in IGF2 Protein and Activation of the PI3K and MAPK Pathways.
IGF2BP3 is known to serve as a translational activator of IGF2 mRNA. Therefore, we determined the expression of IGF2 mRNA and protein in thyroid tumors positive and negative for THADA fusions. Using qRT-PCR, no difference in IGF2 mRNA levels was detected (Fig. 3A). This was confirmed by RNA in situ hybridization (ISH), which showed low levels of IGF2 mRNA in the fusion-positive tumors, similar to those seen in adjacent normal thyroid tissue, in contrast to the high levels of IGF2BP3 mRNA in the fusion-positive tumors (Fig. 3B). However, using Western blotting, a significant increase in IGF2 protein was found in all tested tumors positive for the fusion and IGF2BP3 overexpression (Fig. 3C).
IGF2 is known to act as an autocrine and paracrine regulator of IGF1R (8, 9), leading to activation of the PI3K and MAPK signaling pathways (9, 10). Therefore, we examined whether the increase in IGF2 protein in these human tumor tissues was associated with the increased phosphorylation of AKT and ERK proteins. Using Western blot analysis, we showed increased pAKT and pERK in all tumors positive for the fusion (Fig. 3C). The effect of IGF2BP3 overexpression on IGF2 signaling was further confirmed in vitro. Overexpression of IGF2BP3 in HEK293 cells led to an increase in IGF2 protein, but not mRNA, and increased phosphorylation of AKT and ERK proteins (Fig. 3 D and E). The knockdown experiments were performed in PaCa-2 pancreatic cancer cells, which have a very high level of IGF2BP3 expression, based on the reference database for gene expression analysis (Laboratory for Systems Biology and Medicine) (www.lsbm.org/site_e/database/index.html), which we confirmed by Western blot and immunohistochemistry (IHC) (Fig. 3 F and H). Knockdown of IGF2BP3 using siRNA led to a significant reduction in IGF2 protein levels and decreased pAKT and pERK (Fig. 3 G and H), confirming the impact of IGF2BP3 on translational activation of IGF2 and its downstream signaling.
IGF2BP3 Overexpression Drives Cell Proliferation, Invasion, and Transformation.
To study the biological effect of THADA fusions and IGF2BP3 overexpression in thyroid and other cell types, full-length IGF2BP3 mRNA was overexpressed in FTC133, HEK293, and U2OS cells, all with low levels of endogenous expression of IGF2BP3. Overexpression of IGF2BP3 resulted in a significant increase in proliferation in all three cell lines (Fig. 4 A–C). In a reverse experiment, silencing of IGF2BP3 in PaCa-2 cancer cells, which have high levels of IGF2BP3 expression, led to a significantly reduced growth rate (Fig. 4D).
Next, we explored the impact of IGF2BP3 overexpression on cell migration and invasion. Using a wound-healing assay, IGF2BP3 overexpression in HEK293 cells led to a significantly faster migration (Fig. 4E), whereas IGF2BP3 knockdown in PaCa-2 cells resulted in decreased cell migration (Fig. 4F). Similarly, a Matrigel invasion assay showed that overexpression of IGF2BP3 in HEK293 cells led to a significant enhancement of cell invasion, and knockdown of IGF2BP3 in PaCa-2 cells resulted in reduced cell invasiveness (Fig. 4 G and H).
Finally, we studied the impact of IGF2BP3 overexpression on cell transformation. Using a soft-agar colony-formation assay, FTC133 thyroid cells transfected with IGF2BP3 vector showed a significant increase in colony formation in the soft agar compared with empty vector (Fig. 4I), indicating enhanced anchorage-independent growth, a characteristic of transformed cells. In a reverse experiment, knockdown of IGF2BP3 expression in PaCa-2 cancer cells resulted in a significant reduction of colony formation in soft agar (Fig. 4J). Together, these data indicate that IGF2BP3 overexpression promotes cell proliferation, migration, invasion, and transformation in thyroid and other cell types.
Frequency of IGF2BP3 Overexpression via Gene Fusion and Other Mechanisms in Thyroid and Other Cancers.
To evaluate the rate of IGF2BP3 alterations in thyroid and other tumors, we first developed an IHC assay for specific and sensitive detection of overexpression of IGF2BP3 protein in tumors carrying THADA–LOC389473 and THADA–IGF2BP3 fusions. For this, IHC for IGF2BP3 was titrated using 7 fusion-positive and 18 fusion-negative thyroid cancers to achieve strong and diffuse cytoplasmic immunoreactivity in all positive cancers and complete absence of immunoreactivity in fusion-negative cancers (SI Appendix, Fig. S6A). Further, a targeted next-generation sequencing (NGS) was used for quantitative assessment of IGF2BP3 mRNA. Analysis of 192 PTCs, which represented a series of consecutive tumors with this diagnosis analyzed by targeted NGS, revealed 10 (5.2%) cases with strong IGF2BP3 immunoreactivity; all were confirmed to have high levels of IGF2BP3 mRNA expression (SI Appendix, Fig. S6B). Using RT-PCR, 9 out of the 10 (90%) tumors were positive for a fusion, typically THADA–LOC389473. Rearrangement involving the IGF2BP3 region was further confirmed by FISH in all 10 IHC-positive thyroid cancers (SI Appendix, Fig. S6C). This demonstrated high specificity of the IHC assay in detecting tumors with IGF2BP3 overexpression caused by chromosomal rearrangements. It also showed that chromosomal rearrangement involving THADA fused to a chromosomal region upstream of IGF2BP3 is a dominant mechanism of IGF2BP3 overexpression in PTC.
Next, we studied clinical and phenotypic features of thyroid cancers that showed IGF2BP3 overexpression. These tumors were characterized by slight female predominance (1.5:1), mean patient age of 48.9 y, and tumor size ranging from 1.6 to 9 cm (mean 3.8 cm). Despite the large tumor size, microscopically the tumors were either encapsulated (n = 5) or well-circumscribed (n = 5), with the follicular growth pattern characteristic of the follicular variant of PTC or noninvasive follicular thyroid tumor with papillary-like nuclear features (NIFTPs) (11) (SI Appendix, Fig. S6D). None of them showed other common driver mutations known to occur in thyroid cancer (e.g., BRAF, RAS), providing additional evidence for THADA–LOC389473 and THADA–IGF2BP3 fusions being a driver event in these tumors.
Further, we assessed the frequency of IGF2BP3 overexpression and its mechanisms in other cancer types. Using the IGF2BP3 IHC assay to screen tissue microarrays of 12 common cancer types, we found strong and diffuse immunoreactivity in 14.2% of pancreatic cancers, 12.6% of lung squamous cell carcinomas, 10.1% of lung adenocarcinomas, 10% of gastric cancers, 9.7% of cholangiocarcinomas, 9.1% of bladder cancers, 7.5% of ovarian cancers, and 3.8% of colon cancers (SI Appendix, Fig. S6 E and F and Table S2). Using FISH analysis of selected IHC-positive cancers, chromosomal rearrangements involving the IGF2BP3 region were identified in 3/12 (25%) pancreatobiliary cancers (SI Appendix, Fig. S6 F and G), and IGF2BP3 amplification in 3/18 (16.7%) IHC-positive lung adenocarcinomas, 1/8 (12.5%) pancreatic, and 1/5 (20%) bladder cancers (SI Appendix, Fig. S6 F and H and Table S2). These findings are in agreement with the cancer genome atlas (TCGA) data, where amplification of IGF2BP3 was found in 0 to 7.1% of different cancer types (SI Appendix, Table S3). This suggests that in addition to gene fusions, many nonthyroid cancers have IGF2BP3 overexpression, which is likely due to gene amplifications or other, possibly epigenetic, mechanisms such as aberrant methylation (SI Appendix, Fig. S6I). Overall, these data demonstrate that strong overexpression of IGF2BP3, driven by gene fusions as well as other genetic and possibly epigenetic mechanisms, occurs in a distinct subset of thyroid and other cancer types.
Effects of IGF1R Inhibition on Growth of Cancer Cells Overexpressing IGF2BP3.
The data presented above indicate that IGF2BP3 overexpression via chromosomal rearrangement and other mechanisms occurs in a distinct subset of thyroid and other cancers and results in an increase in IGF2 protein, signaling via IGF1R, and cell proliferation, invasion, and transformation. Increased IGF1R signaling has been linked to elevated cancer risk and more aggressive behavior of different cancers, and pharmacological inhibitors of IGF1R, such as the small-molecule inhibitor OSI-906 (Linsitinib), are available (12, 13). Therefore, we tested the effects of IGF1R inhibition on the growth of cancer cells driven by IGF2BP3 overexpression. First, we studied whether activation of the AKT and MAPK pathways and cell growth induced by IGF2BP3 overexpression are affected by inhibiting IGF1R by OSI-906 in vitro. Western blotting showed that inhibition of IGF1R blocked phosphorylation of AKT, S6, and ERK induced by IGF2BP3 overexpression in HEK293 cells (Fig. 5A). Further, we found that inhibition of IGF1R by OSI-906 led to dose-dependent growth inhibition of PaCa-2 cancer cells, which strongly overexpress IGF2BP3, but did not have any effects on FTC133 and U2OS cells with low levels of IGF2BP3 expression (Fig. 5B). However, when IGF2BP3 expression was induced in FTC133 and U2OS cells by transient transfection, both cell types acquired sensitivity to growth inhibition by OSI-906, and subsequent inhibition of IGF1R returned the cells to an IGF2BP3-negative–like state (Fig. 5C). Similar effects of IGF1R inhibition were observed on migration and invasion of PaCa-2 cells (Fig. 5 D and E). Further, OSI-906 treatment of PaCa-2 cells led to significant reduction of colony formation in soft agar (Fig. 5F).
To determine whether the inhibitory effect of OSI-906 on cell proliferation is mediated by IGF2, we tested whether the inhibition of PaCa-2 cell proliferation following IGF2BP3 knockdown can be rescued by IGF2. Indeed, the reduction in PaCa-2 cell growth by IGF2BP3 siRNA was reversed after supplementing the cell-culture medium with sufficient concentrations of exogenous IGF2 (Fig. 5G).
Next, we explored whether growth of tumor cells overexpressing IGF2BP3 can be blocked via IGF1R inhibition in vivo. We used the pancreatic cancer cells PaCa-2 and colon cancer cells Caco-2, both expressing IGF2BP3 at high levels (Fig. 5B), and the thyroid cancer cells FTC133, with low levels of IGF2BP3 expression, to establish tumors growing in nude mice at the sites of s.c. cell injection. Treatment of mice with therapeutic doses of OSI-906 arrested growth of tumors originating from PaCa-2 and Caco-2 cells (Fig. 5H) but had no effect on the growth of FTC133-derived tumors (Fig. 5I). As expected, the inhibitory effect on tumor growth coincided with the reduction of phosphorylated IGF1R, AKT, S6, and ERK in the tumor tissue (Fig. 5H), whereas tumors originating from FTC133 and lacking IGF2BP3 expression did not show reduction in the phosphorylation of these proteins (Fig. 5I). These data show that growth of cancer cells overexpressing IGF2BP3 can be arrested by IGF1R inhibitors in vitro and in vivo, providing early evidence for a possible benefit of anti-IGF1R therapy in cancers with strong overexpression of IGF2BP3 induced by THADA–LOC389473 fusion or other mechanisms.
Discussion
In this study, we report the discovery and characterization of a recurrent driver event in papillary thyroid cancer. The mutational landscape of this tumor is dominated by point mutations and gene fusions typically affecting genes coding for receptor tyrosine kinases (RTKs) or intracellular effectors propagating signals from RTKs via the MAPK and PI3K pathways (1). Here we report a different class of mutations that indirectly affect the expression of IGF2BP3, a gene coding for the IGF2 mRNA-binding protein that acts as a posttranscriptional regulator of IGF2 and via stimulation of the IGF1 receptor leads to the same end points, namely activation of MAPK and PI3K signaling. The prototypic molecular event leading to IGF2BP3 overexpression discovered in this study is a fusion between the THADA gene locus on 2p21 and a chromosomal region on 7p15 containing the LOC389473 gene located upstream of the IGF2BP3 gene. Chromosomal translocation t(2;7) has been previously reported in thyroid tumors leading to the fusion of THADA to an untranslated region on chromosome 7, prompting the authors to suggest that its biological effects may involve disrupting the function of THADA, a death receptor-interacting protein (14). Here we show that this chromosomal translocation leads to strong overexpression of the IGF2BP3 gene located downstream of the fusion point. We were not able to identify THADA–IGF2BP3 fusion mRNA in the majority of these tumors, and instead observed strong overexpression of the full-length IGF2BP3 mRNA and protein. Although we cannot exclude that precursor fusion mRNA and protein are made, it appears more likely that IGF2BP3 up-regulation is due to the gene juxtaposition to the THADA locus actively transcribed in thyroid cells. Such a mechanism of gene activation, although not common, is known to occur as a consequence of chromosomal rearrangements in other tumor types, particularly in hematological malignancies (5, 15, 16). Our results support the possibility that in virtually all thyroid tumors the fusion on the DNA level occurs upstream of the IGF2BP3 gene. However, this could not be confirmed directly in this study, because in most tumors the fusions could be studied only at the RNA and not at the DNA sequence level.
IGF2BP3 belongs to the family of IGF2-binding proteins that regulate the expression of IGF2 and other proteins by binding to target mRNAs and controlling their stability and translation (17, 18). It is expressed mostly during embryogenesis and retains no or low expression levels in adult tissues (19, 20). IGF2BP3 has been found to be overexpressed in several cancer types and to affect tumor growth, cell transformation, and metastatic spread (18, 21, 22). However, the mechanisms responsible for IGF2BP3 overexpression in those cancers remain poorly understood, with down-regulation of let-7 microRNAs proposed as a possible factor (23). Here we show that THADA–LOC389473 and other THADA fusions upstream of the IGF2BP3 gene represent a previously unknown structural mechanism responsible for IGF2BP3 overexpression in thyroid and other cancer types.
Thyroid tumors positive for THADA fusions demonstrate a distinct phenotype, as they tend to be large in size but fully encapsulated and showing low-grade morphology. In fact, some of these tumors, previously designated papillary carcinomas, may now be reclassified as more indolent, borderline tumors termed NIFTPs (11). However, IGF2BP3 protein overexpression was also observed in a subset of aggressive poorly differentiated thyroid cancers (13). In addition to thyroid tumors, we detected strong IGF2BP3 overexpression by IHC in ∼5 to 15% of other cancer types. Further, we showed that strong overexpression of IGF2BP3 led to posttranscriptional up-regulation of IGF2, activation of IGF1R signaling, and increased cell proliferation, migration, and transformation. Although we cannot exclude that biological effects of IGF2BP3 overexpression may involve not only IGF2 but other mRNA targets, our results provide evidence that IGF2/IGF1R signaling is primarily responsible for biological effects of IGF2BP3 overexpression in these cells.
Our data also suggest that growth of these cells and tumors can be arrested either by blocking IGF2BP3 overexpression or by IGF1R inhibition. This raises the possibility that IGF2BP3 overexpression may serve as a predictor of therapeutic response to IGF1R inhibitors such as OSI-906 (24). Indeed, the IGF1R pathway has been explored as a therapeutic target with either anti-IGF1R antibodies or small-molecule inhibitors. However, most trials showed no clinical benefit of targeting this pathway in patients with advanced cancer (25). In trials that used small-molecule inhibitors, such as OSI-906 (Linsitinib), which binds dually to IGF1R and insulin receptor, clinical response was registered only in a small (1 to 5%) but distinct proportion of patients with solid tumors (24, 26). The results of this study provide early, preclinical evidence that IGF2BP3 overexpression may be used to predict therapeutic response to IGF1R inhibitors in patients with various, frequently highly lethal cancer types, which should be further explored in future studies.
Materials and Methods
Tissue samples were collected and deidentified according to the University of Pittsburgh institutional review board-approved protocol. No patient consent was required. Paired-end whole-genome and whole-exome sequencing was performed on an Illumina HiSeq 2000. Cell-proliferation assays were performed using alamarBlue. Matrigel invasion chambers were used for cell-invasion assays, and soft-agar colony-formation CytoSelect Kits to assess transformation. Detailed methods are provided in SI Appendix, Materials and Methods.
Supplementary Material
Acknowledgments
We thank the University of Pittsburgh Health Sciences Tissue Bank for providing tissue samples. This work was supported by NIH Grant R01 CA88041 (to Y.E.N.) and by a generous gift from Drs. David and Nancy Brent.
Footnotes
Conflict of interest statement: Y.E.N. is a consultant for Quest Diagnostics.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1614265114/-/DCSupplemental.
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