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. 1990 Feb;87(3):1008–1012. doi: 10.1073/pnas.87.3.1008

Twenty Drosophila visual system cDNA clones: one is a homolog of human arrestin.

D R Hyde 1, K L Mecklenburg 1, J A Pollock 1, T S Vihtelic 1, S Benzer 1
PMCID: PMC53399  PMID: 2105491

Abstract

From a group of 436 Drosophila melanogaster cDNA clones, we selected 39 that are expressed exclusively or predominantly in the adult visual system. By sequence analysis, 20 of the clones appear to represent previously unreported distinct cDNAs. The corresponding transcripts are detected in the retina and optic lobes. The genes are scattered throughout the genome, some near mutations known to affect eye function. One of these clones has been identified, by sequence analysis, as the structural gene (Arr) for a Drosophila homolog of human arrestin. Vertebrate arrestin interacts with rhodopsin in phototransduction and has been associated with an autoimmune form of uveitis in primates. The presence of an arrestin homolog in Drosophila suggests that both the vertebrate and invertebrate phototransduction cascades are regulated in a similar manner.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ballinger D. G., Benzer S. Targeted gene mutations in Drosophila. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9402–9406. doi: 10.1073/pnas.86.23.9402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bloomquist B. T., Shortridge R. D., Schneuwly S., Perdew M., Montell C., Steller H., Rubin G., Pak W. L. Isolation of a putative phospholipase C gene of Drosophila, norpA, and its role in phototransduction. Cell. 1988 Aug 26;54(5):723–733. doi: 10.1016/s0092-8674(88)80017-5. [DOI] [PubMed] [Google Scholar]
  3. Feiler R., Harris W. A., Kirschfeld K., Wehrhahn C., Zuker C. S. Targeted misexpression of a Drosophila opsin gene leads to altered visual function. Nature. 1988 Jun 23;333(6175):737–741. doi: 10.1038/333737a0. [DOI] [PubMed] [Google Scholar]
  4. Fryxell K. J., Meyerowitz E. M. An opsin gene that is expressed only in the R7 photoreceptor cell of Drosophila. EMBO J. 1987 Feb;6(2):443–451. doi: 10.1002/j.1460-2075.1987.tb04774.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kozak M. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984 Jan 25;12(2):857–872. doi: 10.1093/nar/12.2.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kühn H., Hall S. W., Wilden U. Light-induced binding of 48-kDa protein to photoreceptor membranes is highly enhanced by phosphorylation of rhodopsin. FEBS Lett. 1984 Oct 29;176(2):473–478. doi: 10.1016/0014-5793(84)81221-1. [DOI] [PubMed] [Google Scholar]
  7. Levy L. S., Ganguly R., Ganguly N., Manning J. E. The selection, expression, and organization of a set of head-specific genes in Drosophila. Dev Biol. 1982 Dec;94(2):451–464. doi: 10.1016/0012-1606(82)90362-1. [DOI] [PubMed] [Google Scholar]
  8. Maniatis T., Hardison R. C., Lacy E., Lauer J., O'Connell C., Quon D., Sim G. K., Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. doi: 10.1016/0092-8674(78)90036-3. [DOI] [PubMed] [Google Scholar]
  9. Matsumoto H., Pak W. L. Light-induced phosphorylation of retina-specific polypeptides of Drosophila in vivo. Science. 1984 Jan 13;223(4632):184–186. doi: 10.1126/science.6419348. [DOI] [PubMed] [Google Scholar]
  10. Mismer D., Michael W. M., Laverty T. R., Rubin G. M. Analysis of the promoter of the Rh2 opsin gene in Drosophila melanogaster. Genetics. 1988 Sep;120(1):173–180. doi: 10.1093/genetics/120.1.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mismer D., Rubin G. M. Definition of cis-acting elements regulating expression of the Drosophila melanogaster ninaE opsin gene by oligonucleotide-directed mutagenesis. Genetics. 1989 Jan;121(1):77–87. doi: 10.1093/genetics/121.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Montell C., Jones K., Hafen E., Rubin G. Rescue of the Drosophila phototransduction mutation trp by germline transformation. Science. 1985 Nov 29;230(4729):1040–1043. doi: 10.1126/science.3933112. [DOI] [PubMed] [Google Scholar]
  13. Montell C., Jones K., Zuker C., Rubin G. A second opsin gene expressed in the ultraviolet-sensitive R7 photoreceptor cells of Drosophila melanogaster. J Neurosci. 1987 May;7(5):1558–1566. doi: 10.1523/JNEUROSCI.07-05-01558.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Montell C., Rubin G. M. The Drosophila ninaC locus encodes two photoreceptor cell specific proteins with domains homologous to protein kinases and the myosin heavy chain head. Cell. 1988 Mar 11;52(5):757–772. doi: 10.1016/0092-8674(88)90413-8. [DOI] [PubMed] [Google Scholar]
  15. Nathans J., Hogness D. S. Isolation and nucleotide sequence of the gene encoding human rhodopsin. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4851–4855. doi: 10.1073/pnas.81.15.4851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. O'Tousa J. E., Baehr W., Martin R. L., Hirsh J., Pak W. L., Applebury M. L. The Drosophila ninaE gene encodes an opsin. Cell. 1985 Apr;40(4):839–850. doi: 10.1016/0092-8674(85)90343-5. [DOI] [PubMed] [Google Scholar]
  17. Palazzolo M. J., Hyde D. R., VijayRaghavan K., Mecklenburg K., Benzer S., Meyerowitz E. Use of a new strategy to isolate and characterize 436 Drosophila cDNA clones corresponding to RNAs detected in adult heads but not in early embryos. Neuron. 1989 Oct;3(4):527–539. doi: 10.1016/0896-6273(89)90211-0. [DOI] [PubMed] [Google Scholar]
  18. Paulsen R., Bentrop J. Activation of rhodopsin phosphorylation is triggered by the lumirhodopsin-metarhodopsin I transition. 1983 Mar 31-Apr 6Nature. 302(5907):417–419. doi: 10.1038/302417a0. [DOI] [PubMed] [Google Scholar]
  19. Pollock J. A., Benzer S. Transcript localization of four opsin genes in the three visual organs of Drosophila; RH2 is ocellus specific. Nature. 1988 Jun 23;333(6175):779–782. doi: 10.1038/333779a0. [DOI] [PubMed] [Google Scholar]
  20. Schaeffer E., Smith D., Mardon G., Quinn W., Zuker C. Isolation and characterization of two new drosophila protein kinase C genes, including one specifically expressed in photoreceptor cells. Cell. 1989 May 5;57(3):403–412. doi: 10.1016/0092-8674(89)90915-x. [DOI] [PubMed] [Google Scholar]
  21. Shieh B. H., Stamnes M. A., Seavello S., Harris G. L., Zuker C. S. The ninaA gene required for visual transduction in Drosophila encodes a homologue of cyclosporin A-binding protein. Nature. 1989 Mar 2;338(6210):67–70. doi: 10.1038/338067a0. [DOI] [PubMed] [Google Scholar]
  22. Shinohara T., Dietzschold B., Craft C. M., Wistow G., Early J. J., Donoso L. A., Horwitz J., Tao R. Primary and secondary structure of bovine retinal S antigen (48-kDa protein). Proc Natl Acad Sci U S A. 1987 Oct;84(20):6975–6979. doi: 10.1073/pnas.84.20.6975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith D. P., Shieh B. H., Zuker C. S. Isolation and structure of an arrestin gene from Drosophila. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1003–1007. doi: 10.1073/pnas.87.3.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wilden U., Hall S. W., Kühn H. Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments. Proc Natl Acad Sci U S A. 1986 Mar;83(5):1174–1178. doi: 10.1073/pnas.83.5.1174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yamaki K., Tsuda M., Shinohara T. The sequence of human retinal S-antigen reveals similarities with alpha-transducin. FEBS Lett. 1988 Jul 4;234(1):39–43. doi: 10.1016/0014-5793(88)81298-5. [DOI] [PubMed] [Google Scholar]
  26. Zuker C. S., Cowman A. F., Rubin G. M. Isolation and structure of a rhodopsin gene from D. melanogaster. Cell. 1985 Apr;40(4):851–858. doi: 10.1016/0092-8674(85)90344-7. [DOI] [PubMed] [Google Scholar]
  27. Zuker C. S., Montell C., Jones K., Laverty T., Rubin G. M. A rhodopsin gene expressed in photoreceptor cell R7 of the Drosophila eye: homologies with other signal-transducing molecules. J Neurosci. 1987 May;7(5):1550–1557. doi: 10.1523/JNEUROSCI.07-05-01550.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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