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. 1969 May;63(1):138–143. doi: 10.1073/pnas.63.1.138

STRAND-SPECIFIC TRANSFER OF DONOR DNA DURING CONJUGATION IN E. coli*

Garret Ihler 1, W Dean Rupp 1,†
PMCID: PMC534013  PMID: 4897022

Abstract

After conjugation of E. coli, only the preformed donor DNA strand with a leading 5′ end can be recovered from the recipient. DNA that was transferred to recipients from radioactive-labeled donor strains lysogenic for λ bacteriophage was recovered as radioactive material in mature phage particles. The radioactivity was recovered in strand l when gene transfer was in the order gal-λ-bio, and in strand r when the order of transfer was bio-λ-gal. The assignment of the 5′ end as the origin of the transferred strand follows directly from the known orientation of prophage λ in the chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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