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. 1969 May;63(1):152–159. doi: 10.1073/pnas.63.1.152

SEPARATION OF MICROBIAL DEOXYRIBONUCLEIC ACIDS INTO COMPLEMENTARY STRANDS*

Rivka Rudner 1,2,3, John D Karkas 1,2,3, Erwin Chargaff 1,2,3
PMCID: PMC534015  PMID: 4980029

Abstract

DNA preparations from seven bacterial species and from the E. coli phage T4 can, after denaturation with alkali, be separated chromatographically into two distinct components (L and H) through intermittent gradient elution from methylated albumin kieselguhr columns. The direct chemical analysis of the L and H fractions isolated from DNA specimens of the AT type shows them to exhibit a high degree of complementarity; but despite a bias in the distribution of purines and pyrimidines, either fraction contains equimolar quantities of 6-amino and of 6-keto nucleotides. In the L and H components derived from DNA of the equimolar and GC types, the distribution bias appears limited to guanine and cytosine. It is suggested that the L and H fractions represent the complementary DNA strands.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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