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. 2016 Oct 15;7(47):77457–77467. doi: 10.18632/oncotarget.12681

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Copyright: © 2016 Di et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Immunofluorescent staining for LONP1 (green) and CM-H₂XRos (red) in D-54 and U-251 cells. Nuclei were counterstained with DAPI (blue). B. D-54 cells had a higher Lon expression than U-251 cells at both protein and mRNA levels. C. Successful knockdown of Lon mRNA was detected in D-54 cells even at Day 7 after siLon-1 transfection. SiRNAs targeting Lon (siLon-1and siLon-2) significantly decreased Lon mRNA levels in U-251 cells 5 days after transfection. D. D-54 and U-251 cells were transfected by siLon-1. Cells were collected and Western blot was used to detect LONP1 at 3 and 7 days after transfection.