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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 May;63(1):86–92. doi: 10.1073/pnas.63.1.86

ISOLATION OF ADENYL CYCLASE FROM Escherichia coli*

Mariano Tao 1,, Fritz Lipmann 1
PMCID: PMC534038  PMID: 4897910

Abstract

We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg++ requirement and is inhibited by fluoride and inorganic pyrophosphate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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