Abstract
We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg++ requirement and is inhibited by fluoride and inorganic pyrophosphate.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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