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. 2016 Dec 21;8(2):1972–1982. doi: 10.18632/oncotarget.14081

Figure 3. JMJD7-PlA2G4B promotes cell survival and AKT activation in HNSCC cells.

Figure 3

A. Apoptosis detection with Annexin V and pI using flow cytometry for control, JMJD7-only, or JMJD7-PLA2G4B KD SCC23 and SCC1 cells. Cells were serum-starved 24 hours prior to the detection to elicit cell death. B. Western blotting of cleaved PARP in SCC23 and SCC1 cells following control, siJMJD7-1, or siJMJD7-4 transfection. C. Western blotting of phosphorylated AKT (p-AKT), AKT, and JMJD7 in SCC1 cells following control, siJMJD7-1, siJMJD7-2, siJMJD7-3, or siJMJD7-4 transfection. D. Western blotting of HGF-induced AKT phosphorylation in SCC1 cells following control, siJMJD7-1, or siJMJD7-4 transfection.