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. 2016 Aug 20;7(41):66647–66659. doi: 10.18632/oncotarget.11336

Figure 1. ARC was expressed in the cochlear HCs and HEI-OC-1 cells.

Figure 1

(A) Immunofluorescence staining showed that ARC was specifically expressed in the HCs, but not in the supporting cells of the organ of Corti in the P7 mice. Myosin 7a and Sox2 were used as markers for HCs and supporting cells, respectively. (B) Immunofluorescence staining showed that ARC was expressed in HEI-OC-1 cells. (C) RT-qPCR showed that ARC was expressed in the cochlea and in HEI-OC-1 cells. Brain samples were used as positive controls, and GAPDH served as a loading control in each lane. (D) Western blot showed that ARC was expressed in the cochlea and HEI-OC-1 cells. β-actin served as a loading control in each lane. Scale bars = 20 μm.