Skip to main content
. 2016 Aug 20;7(41):66647–66659. doi: 10.18632/oncotarget.11336

Figure 3. The ARC level in HEI-OC-1 cells decreased after neomycin injury.

Figure 3

The HEI-OC-1 cells were treated with 1 mM to 10 mM neomycin for 24 h, cultured in serum-free medium for an additional 24 h, then harvested for RT-qPCR and western blot. (A–C) The mRNA and protein levels of ARC in HEI-OC-1 cells decreased gradually with the increasing neomycin doses. (D–F) The mRNA and protein levels of ARC in HEI-OC-1 cells decreased with the increasing neomycin treatment time. (G and H) Immunofluorescence showed that the expression of ARC in TUNEL-positive cells was lower than in TUNEL-negative cells. The HEI-OC-1 cells were cultured with 10 mM neomycin for 24 h. TUNEL-positive cells were defined as apoptotic cells. Data are shown as mean ± S.D. *p < 0.05. Scale bars = 20 μm.