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. 2016 Sep 14;7(45):72807–72818. doi: 10.18632/oncotarget.12020

Figure 5. SASH1 depletion partially rescues chloropyramine-induced apoptosis in breast cancer cell lines.

Figure 5

(A) Cells were transfected with negative control or SASH1 esiRNAs. After 72 h, cell lysates were prepared and SASH1 expression was analysed relative to β-actin by immunoblotting. Knockdown (KD). (B–D) Cells were transfected as above, and chloropyramine was added 24 h post-transfection. Cultures were imaged by light microscopy IncuCyte ZOOM system and digitally analysed to assess confluence relative to the untreated control at 96 h post-treatment. Data shown are means +/− the standard deviation from three independent experiments. t-tests were used to compare cell confluence with and without SASH1 depletion at each of the chloropyramine doses; *p < 0.05.