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. 2016 Sep 29;7(45):73414–73431. doi: 10.18632/oncotarget.12337

Figure 6. CHKα knockdown reduces the viability, invasiveness and clonogenicity of GBM cells.

Figure 6

(A) Knockdown of CHKα reduces the viability of LN229 and GBM1 cells. Exponential growth curves were calculated for each condition and displayed in the graphs. (B) Cells with impaired CHKα expression are less invasive as assessed with modified Boyden chamber assays for 24 h. Representative pictures of hematoxylin stained invaded cells and quantifications of three Boyden chamber experiments are shown (*p < 0.05). (C) CHKα suppression reduces the clonogenicity of JHH520 and GBM1 cells as assessed by soft agar assays. Representative pictures of NBT stained colonies and quantifications of three colony forming assays are shown (*p < 0.05). Abbreviations: HPF, high power field; NBT, 4-Nitro blue tetrazolium chloride. The data is represented as mean ± SD (n = 3).