Table 2. Caspase activation by recombinant VACVs.
Virus titer (PFU/cell) | Time, h | Activated caspases (%) | |
---|---|---|---|
VV-GMCSF-dGF | VV-GMCSF-Lact | ||
0.05 | 12 | 0.2 ± 0.17 | 1.8 ± 0.9 |
24 | 6.6 ± 2.1** | 14.8 ± 1.8** | |
36 | 27.4 ± 3.5** | 37.3 ± 2.3** | |
0.5 | 12 | 3 ± 1.1 | 5.8 ± 1.7 |
24 | 16.5 ± 2.4** | 21.3 ± 1.8** | |
36 | 31.9 ± 2.8 | 35.1 ± 3.1 |
MDA-MB-231 cells were incubated with recombinant VACVs, and the cells with active caspase −3, and −7 were analyzed using flow cytometry as described in the Methods. The percentage of the cells with the active caspase −3 and −7 was calculated as a difference of FAM-positive cells between experimental sample and control sample. The data are presented as a mean of three experiments.
The difference between groups was statistically significant at p<0.05.