Table 2. Caspase activation by recombinant VACVs.

Virus titer (PFU/cell)	Time, h	Activated caspases (%)
		VV-GMCSF-dGF	VV-GMCSF-Lact
0.05	12	0.2 ± 0.17	1.8 ± 0.9
	24	6.6 ± 2.1**	14.8 ± 1.8**
	36	27.4 ± 3.5**	37.3 ± 2.3**
0.5	12	3 ± 1.1	5.8 ± 1.7
	24	16.5 ± 2.4**	21.3 ± 1.8**
	36	31.9 ± 2.8	35.1 ± 3.1

MDA-MB-231 cells were incubated with recombinant VACVs, and the cells with active caspase −3, and −7 were analyzed using flow cytometry as described in the Methods. The percentage of the cells with the active caspase −3 and −7 was calculated as a difference of FAM-positive cells between experimental sample and control sample. The data are presented as a mean of three experiments.

^**The difference between groups was statistically significant at p<0.05.