Table 2. Regulation of selected genes in Aspergillus fumigatus in the presence or absence of human NK cells.
| A. fumigatus | gene expression | |||
|---|---|---|---|---|
| gene (molecule) | x-fold change | 2−ΔΔ Ct; Af/Af+NK | P | |
| Heat shock response | ||||
| hsp70 (heat shock protein 70) | +1.1 | 0.9 ± 0.1/1.0 ± 0.1 | ns | |
| hsp90 (heat shock protein 90) | +1.6 | 1.0 ± 0.1/1.6 ± 0.1 | 0.007 | |
| High affinity iron assimilation | ||||
| freB (ferric chelate reductase) | +1.6 | 0.9 ± 0.0/1.4 ± 0.1 | 0.001 | |
| Proteases/Peptidases | ||||
| alp1 (alkaline protease 1) | +2.1 | 0.9 ± 0.0/1.9 ± 0.3 | 0.01 | |
| dppIV (dipeptidyl peptidase IV) | +1.5 | 0.6 ± 0.1/0.9 ± 0.1 | ns | |
| dppV (dipeptidyl peptidase V) | ±1.0 | 1.3 ± 0.2/1.3 ± 0.2 | ns | |
| ROSa detoxification | ||||
| sod1 (superoxide dismutase 1) | ±1.0 | 0.9 ± 0.2/0.9 ± 0.2 | ns | |
| sod2 (superoxide dismutase 2) | +1.4 | 0.9 ± 0.2/1.3 ± 0.3 | ns | |
| sod3 (superoxide dismutase 3) | +1.1 | 1.5 ± 0.0/1.6 ± 0.2 | ns | |
| cat1 (catalase 1) | ±1.0 | 0.6 ± 0.1/0.6 ± 0.1 | ns | |
| cat2 (catalase 2) | +1.3 | 1.3 ± 0.0/1.7 ± 0.4 | ns | |
| cycA (cytochrome C) | ±1.0 | 0.8 ± 0.1/0.8 ± 0.2 | ns | |
| Mycotoxins | ||||
| mgl (mitogillin) | +1.6 | 0.7 ± 0.1/1.1 ± 0.2 | ns | |
| gliT (gliotoxin) | +1.2 | 0.7 ± 0.1/1.1 ± 0.2 | ns | |
a
ROS = reactive oxygen species
Relative change of the mRNA expression of the gene of interest after 8 hours of (co-) incubation relative to the housekeeping gene and to time point at hour 0, respectively. The first value represents the results in A. fumigatus incubated alone (Af), the second value the results in A. fumigatus incubated with NK cells (Af+NK) (mean±SEM each). Negative values of “x-fold change” indicate down-regulation, positive values up-regulation. Shown are the results of at least five independent experiments.