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. 2016 Aug 2;7(34):54274–54289. doi: 10.18632/oncotarget.11026

Figure 2. DsRed expression resulted in aggregation and cardiac myopathy.

Figure 2

A. Representative H&E staining of the heart sections isolated from 2-month-old (2M) and 5-month-old (5M) WT, CALELD(+/+), RFP(+/−) and RFP(+/+) mouse. Scale bar = 20 μm. B. RFP immunostaining (shown in red by using Alexa Fluor 568-conjugated secondary Ab) and DAPI nuclear staining (blue) in 2-month-old wild-type and RFP heart sections showing large RFP clusters (asterisks) in RFP+/− heart. Both RFP clusters (asterisks) and additional smaller aggregates (arrows) were present in RFP+/+ heart. Scale bar = 20 μm. C. Native PAGE and immunoblot using anti-RFP Ab shows the banding pattern of DsRed oligomers in LV of RFP mice. Molecular weights (in kDa) are indicated on the left, and asterisks indicate major DsRed oligomers. MW of DsRed monomer is approximately 28 kDa. D. Immunostaining of RFP (red), Connexin 43, (green) and DAPI (blue) of 2- month-old wild-type and RFP+/+ heart. Scale bar =25 μm.