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. 2016 Jul 19;7(34):54883–54896. doi: 10.18632/oncotarget.10702

Figure 1. Combination treatment with γIR and PI-103 causes the loss of stemness markers and FoxO proteins in p53-proficient GBM-SCs.

Figure 1

p53-proficient GBM-SCs (A) or p53-deficient GBM-SCs (B) were incubated with 0.5 μM PI-103 for 1 h and then irradiated. Samples were analyzed by Western blot at the time points indicated. Actin served as loading control. Shown are representative results from 4 (GBM22), 2 (GBM36), 2 (GBM10), 3 (GBM4), and 2 (G166) experiments, respectively. (C) Quantification of changes in the expression of stem and progenitor markers and of FoxO proteins after combination treatment with 10 Gy + PI-103 compared to untreated cells; black columns: p53-proficient GBM-SCs; open columns: p53-deficient GBM-SCs. (D) Single cells with functional or non-functional p53 were treated for 1 h with 0.5 μM PI-103 and then irradiated. Three days later, the cells were analyzed for sphere formation. n = 3 experiments. The bar represents 500 μm.