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. 2016 Mar 14;7(43):69397–69411. doi: 10.18632/oncotarget.8043

Figure 1. AR and Thr199 and Thr234/237 NPM1 localized in sub-nuclear structures.

Figure 1

A and B. LNCaP and VCaP cells were starved for 24 hours and then stimulated with 10 nmol/L DHT for 1 hour. Cells were fixed with methanol, immunostained (NPM1, AR and Thr199 and Thr234/237 NPM1) and analyzed by spinning disk fluorescence microscopy. Residual blurring was removed by spatial deconvolution. Nuclei were stained with DAPI. Scale bars, 2.5 μm. White arrows show the co-immunofluorescences and orange arrows show nucleoli. C. Nuclei of stimulated LNCaP were purified and AR was immuno-precipitated. The presence of NPM1 and its two phosphorylated forms were revealed by Western blotting.