Figure 4. N6L inhibited human prostate tumor cell growth in vitro and in vivo.

A and B. Viability of cells treated for 72 hours with incremental N6L concentrations was evaluated using MTT staining in androgen sensitive (A) or independent (B) cells. C. Effect of the combination of N6L and androgen deprived (AD) condition. LNCaP cells were treated for 24 hours with N6L or with PBS and then, incubated in complete medium or in red phenol free RPMI, 10% cs-FBS (AD, mimicking hormonotherapy) for 48 hours. Cell viability was measured using MTT staining. The N6L curve represents the effect of N6L alone, the N6L + AD curve represents the effect of the combination N6L and AD and the N6L + AD normalized curve represents the effect of the combination N6L and AD with a 100% of cell viability corresponding to hormonotherapy treated cells without N6L treatment. D. Effect of the N6L/castration combination in vivo in VCaP ectopic xenografts in nude mice. Mice bearing tumors of about 600 mm³ were castrated. Treatments were performed three times per week by intra-peritoneal injections of PBS (Control group, black curve) or 10 mg/kg N6L one week before castration (BC, blue curve), one day after castration (AC, red curve) or during tumor regrowth (RG, green curve) (n = 8 per group).