Figure 7. Increased cisplatin sensitivity leading to apoptosis after uPAR knockdown in rat MM cells.

(A) Relative cell viability by MTT assay 24 h after exposure to each concentration of cisplatin with uPAR knockdown in EM and SM cells. (B) Flow cytometry analysis and (C) its quantitation for increased early (AnnexinV^high/PI^low) and late (AnnexinV^high/PI^high) apoptosis with uPAR knockdown and rescued by myr-AKT transduction. (D) Caspase activation observed by cleaved caspase 3 in EM and SM cells via Western blot analysis after uPAR knockdown and rescued by myr-AKT transduction. EM, epithelioid subtype mesothelioma; SM, sarcomatoid subtype mesothelioma; CDDP, cisplatin (means ± SEM; *P < 0.05, **P < 0.01). See text for details.