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. 2016 Sep 7;7(43):70404–70419. doi: 10.18632/oncotarget.11879

Figure 5. Histology of NHPrE1/EV and NHPrE1/AR grafts.

Figure 5

IHC stains for cytokeratin 8/18 (ck8/18, luminal epithelial cell marker), p63 (basal epithelial cell marker), and ki67 (cell proliferation marker) were performed on serial sections derived from NHPrE1/EV A, B, E, F, I, and J. or NHPrE1/AR grafts C, D, G, H, K, and L. B, D, F, H, J, and L are higher magnification photomicrographs of A, C, E, G, I, and K, respectively. Arrows in panels C, G, and K indicate host kidney. While NHPrE1/EV control cells formed glandular structures consisting of cytokeratin 8/18-positive luminal epithelial cells (A and B) and p63-positive basal cells (E and F), NHPrE1/AR cells formed invasive carcinomas that were positive for both cytokeratin 8/18 (C and D) and p63 IHC staining (G and H). Ki67 was barely detectable in luminal epithelial cells from NHPrE1/EV grafts (I and J), but was present in many basal cell nuclei in NHPrE1/EV grafts (I and J), as well as in malignant cells in NHPrE1/AR grafts (K and L). Scale bars represent 25 μm.